检验医学与临床Issue(1)：31-32,34,3.DOI:10.3969/j.issn.1672-9455.2013.01.014
106株铜绿假单胞菌耐药性分析及金属β-内酰胺酶检测
Drug resistance analysis of 106 strains of Pseudomonas aeruginosa and detection of metallo-β-lactamases
王芬 1王朝晖 1邰宏明 1陈志1
作者信息
- 1. 江苏省兴化市人民医院检验科 225700
- 折叠
摘要
Abstract
Objective To analyze distribution ,drug resistance and situation of producing metallo-β-lactamase of hospital Pseudomonas aeruginosa for providing the experimental basis of the rational use of drugs for clinical treat-ment of Pseudomonas aeruginosa infection .Methods 106 stains of P .aeruginosa were identified by VITEK-2 auto-matic identification system ,and ,in vitro drug resistance and statistical resistance rates were tested by K-BETA disk diffusion method ,while the double disk synergy assay was used to test the percentage of metallo-β-lactamase .Results In 106 stains of Pseudomonas aeruginosa ,22 (20 .8% )of them produced metallo-β-lactamase .Metallo-β-lactamase positive strains of Pseudomonas aeruginosa had significantly higher drug resistance rates to ceftazidime (CAZ ) , cefepime(FEP ) ,imipenem (IPN ) ,cefoperazone/Sulbactam (SCF ) ,piperacillin (PIP ) ,piperacillin/tazobactam (the PIT ) ,ceftriaxone(CRO) ,cefoperazone(CFP) and than negative strains (P<0 .05) .There wa no significant difference of drug resistance rates of them to Aztreonam (AZT ) ,levofloxacin(LVF) ,gentamicin(GM ) (P>0 .05) .Conclusion Producing metallo-β-lactamase of Pseudomonas aeruginosa is one of the main mechanism of drug resistance to the enzyme β-lactam antimicrobial agents ,it should be rationally use medicines in order to reduce resistance strains generating .关键词
铜绿假单胞菌/金属β-内酰胺酶/耐药性Key words
Pseudomonas aeruginosa/metallo-β-lactamase/resistance引用本文复制引用
王芬,王朝晖,邰宏明,陈志..106株铜绿假单胞菌耐药性分析及金属β-内酰胺酶检测[J].检验医学与临床,2013,(1):31-32,34,3.
