检验医学与临床Issue(16)：2100-2101,2.DOI:10.3969/j.issn.1672-9455.2013.16.018
酶联免疫吸附法检测血清舒芬太尼血药浓度的应用价值
Application value of the enzyme-linked immunosorbent assay in plasma concentration of sufentanil
王宗杰 1黄卫彤 1廖旺1
作者信息
- 1. 广西壮族自治区南宁市妇幼保健院检验科 530011
- 折叠
摘要
Abstract
Objective To investigate value of enzyme-linked immunosorbent assay (ELISA ) quantitative de-tection serum sufentanil concentration ,analgesia delivery and the plasma concentrationcord in cord blood .Methods Used the two step methed of ELISA testing the plasma concentration of sufentanil ,fitting the standard curve to de-termine its detection limit ,and detect the plasma concentration of 48 samples which from the automatic control of in-traspinal analgesia and cord blood ,detect the plasma concentration when 1 hour ,3 hours ,5 hours andplasma concen-tration of cord blood .Results The method draws the sufentanil standard curve :Y = 420 .375X+ 32 .043 ,1 hours plasma concentration of sufentanil was (0 .087 ± 0 .013 )μg/mL ,3 hours plasma concentration of sufentanil was (0 .026 ± 0 .011)μg/mL ,5 hours plasma concentration of sufentanil was (0 .011 ± 0 .050)μg/mL ,The cord blood plasma concentration was (0 .040 ± 0 .029)μg/mL .Conclusion ELISA method for determination of serum sufentanil is simple ,fast .Ideal for labor analgesia ,it could be suitable for clinical pharmacokinetic study .umbilical cord blood-drug concentration in very small amounts and no adverse drug reaction .关键词
舒芬太尼/酶联免疫吸附法/血药浓度/脐带血Key words
sufentanil/enzyme-linked immunosorbent assay/plasma concentration/cord blood引用本文复制引用
王宗杰,黄卫彤,廖旺..酶联免疫吸附法检测血清舒芬太尼血药浓度的应用价值[J].检验医学与临床,2013,(16):2100-2101,2.
