检验医学与临床Issue(20)：3040-3043,4.DOI:10.3969/j.issn.1672-9455.2015.20.027
重度子痫前期患者外周血Th17和Treg细胞及相关细胞因子的表达及意义
Expression and significance of peripheral blood Th 17,Treg cells and related cytokines in patients with severe preeclamp-sia
摘要
Abstract
Objective To investigate the changes and signifcance of peripheral blood Th17 ,CD4+CD25+ iTreg cells and the related cytokines (IL‐17 ,IL‐21 ,IL‐10 ) and TGF‐βin the patients with severe preeclampsia (sPE) .Meth‐ods 30 sPE patients(sPE group) and 30 cases of normal pregnancy(control group) were selected as the research subjects .The levels of Th17 and CD4+CD25+ Treg cells in peripheral blood were detected by flow cytometry .QRT‐PCR was used to detect the mRNA expression level of RORγt and Foxp3 .Plasma cytokines levels of IL‐17 ,IL‐21 ,IL‐10 and TGF‐βwere measured by ELISA .Results The peripheral blood Th17 cells level in the sPE group was signif‐cantly higher than that in the control group ,the difference was statistically significant [(1 .16 ± 0 .78)% vs .(0 .68 ± 0 .35)% ,t=3 .447 ,P=0 .002] .The level of peripheral blood CD4+ CD25+ Treg cells in the sPE group was lower than that in the control group ,the difference was statistically significant [(1 .65 ± 0 .49)% vs .(2 .42 ± 0 .91)% , t= -4 .25 ,P=0 .000] .The expressions level of RORγt mRNA in the sPE group was higher than that in the control group ,the difference was statistically significant [(0 .190 ± 0 .063) vs .(0 .136 ± 0 .037) ,t= 3 .776 ,P=0 .001] ,but the expression level of Foxp3 mRNA was decreased compared with the control group ,the difference was statistically significant[(0 .069 ± 0 .025) vs .(0 .127 ± 0 .027) ,t= -8 .218 ,P=0 .000] .Plasma levels of IL‐17 ,IL‐21 and IL‐10 in the sPE group were (82 .03 ± 37 .61) ,(63 .29 ± 27 .42) ,(56 .89 ± 17 .28)ng/L respectively ,which were higher than (30 .81 ± 21 .87) ,(33 .87 ± 13 .73) ,(33 .24 ± 11 .93) ng/L in the control group respectively ,the differences were sta‐tistically significant(P<0 .01) .However ,the TGF‐β level in the sPE group was markedly decreased compared with the control group ,the difference was statistically significant [(24 .34 ± 6 .51)ng/L vs .(46 .97 ± 15 .81)ng/L ,t= -7 .781 ,P=0 .000] .Plasma IL‐17 and IL‐21 levels in the sPE patients were positively correlated with the Th 17 cells level(r=0 .695 ,0 .586 ,P<0 .01) .Conclusion Th17 cells increase ,CD4+CD25+ Treg cells decrease and related cytokines disorder in the sPE patients could play an important role in the pathogenesis of sPE .关键词
先兆子痫/T淋巴细胞/细胞因子类Key words
preeclampsia/T-lymphocytes/cytokines引用本文复制引用
汪勤,赵春辉,夏良萍,朱华英..重度子痫前期患者外周血Th17和Treg细胞及相关细胞因子的表达及意义[J].检验医学与临床,2015,(20):3040-3043,4.基金项目
江苏省镇江市科技计划项目（SH2013061、SH2014063）。 （）
