摘要
Abstract
Objective To study the stability of cytomegalovirus (CMV) DNA in plasma after 30 d storage un‐der 4 ℃ .Methods The plasma samples from 150 patients undergoing hematopoietic stem cell transplantation were selected and divided into 3 groups according to DNA loads of human CMV :low load group(n=50 ,CMV DNA loads 300-1 000 copy/mL) ,middle load group(n=50 ,CMV DNA load 1 000 -10 000 copy/mL) and high loads group (n=50 ,CMV DNA loads 10 000-100 000 copy/mL) .The base line load(0 d) detection was completed within 2 d after sample collection ,the other palasma was stored under the condition of 4 ℃ .The human CMV load was detected on 1 ,2 ,3 ,7 ,14 ,30 d storage .Results The 150 samples were matched for different time points ,cytomegalovirus loads of 1 ,2 ,3 ,7 ,14 ,21 ,30 days compared with baseline ,the difference had no statistical significance (P>0 .05) .Conclu‐sion The plasma sample after 30 d storage under 4 ℃ has no influence on the detection results of CMV load .关键词
保存时间/血浆/标本/人巨细胞病毒Key words
storage time/plasma/sample/human cytomegalovirus
