检验医学与临床Issue(21)：3140-3143,4.DOI:10.3969/j.issn.1672-9455.2015.21.004
医院获得性尿路感染肠球菌耐药性与毒力基因型相关研究
Correlation of drug resistance with virulence genotypes of Enterococcus in hospital acquired urinary tract infection
摘要
Abstract
Objective To investigate the distribution of virulence factors and drug resistance of Enterococcus in hospital‐acquired urinary tract infections ,and their correlation to provide the basis for rationally using antibacterial drugs and controlling infection .Methods 74 strains of Enterococcus isolated from clinical specimens of urinary tract infection were performed the bacterial strain identification and the antimicrobial susceptibility test was conducted by the K‐B method .Virulence genes of cylL‐L ,cylL‐S ,cylL‐A ,esp ,acm ,gelE ,asa‐I ,cpd and ace were detected by PCR . Results Among 74 strains of enterococcal ,45(60 .8% ) strains and 29(39 .2% ) strains were E .faecalis and E .faeci‐um ,respectively .The resistance rates of Enterococci isolates to penicillin G ,ampicillin and ciprofloxacin were 89 .66% ,89 .66% and 79 .3% ,which were significantly higher than that of E .faecalis .Among E .faecalis ,the resist‐ance rate was 46 .67% to gentamicin ,53 .33% to tetracycline ,which were slightly higher than that of E .faecium .The positive rates of cylL‐L ,cylL‐S ,cylL‐A ,esp ,acm ,gelE ,asa‐1 ,cpd ,ace were 24 .32% ,44 .59% ,22 .97% ,44 .59% , 60 .81% ,22 .97% ,25 .67% ,22 .97% and 9 .46% ,respectively .A significant correlation was found between distribu‐tion of virulence genes and antimicrobial resistance of E .faecium(P<0 .05) .Conclusion E .faecalis is the predomi‐nant species in hospital‐acquired urinary infection ;the majority of E .faecium was multidrug‐resistant ;E .faecalis has more virulence genes than E .faecium .There is a close relationship between the virulence genes and the drug resist‐ance .Thus it is necessary for clinic to pay attention to the reasonable use of antibacterial drugs so as to prevent the e‐mergence and spread of Enterococcus .关键词
尿路感染/屎肠球菌/粪肠球菌/毒力基因/耐药性Key words
urinary tract infections/E .faecium/E .faecalis/virulence gene/drug resistance引用本文复制引用
黄小丽,赵瑞珂,李艳萌,余佳佳,张险峰,钱雪峰,韩清珍,徐杰..医院获得性尿路感染肠球菌耐药性与毒力基因型相关研究[J].检验医学与临床,2015,(21):3140-3143,4.基金项目
江苏省卫生厅医学科研项目（Q201401）；江苏省研究生培养创新工程项目（KYLX-1261）；苏州市“科教兴卫”青年课题项目（kjx w 2014008）。 （）
