检验医学与临床Issue(21)：3217-3218,3220,3.DOI:10.3969/j.issn.1672-9455.2015.21.033
高脂血、溶血标本对荧光定量聚合酶链反应测定低水平HBV-DNA的影响
Influence of hyperlipemia and hemolysis specimens on fluorescence quantitative PCR determination of low level HBV-DNA
刘小敏 1唐恒锋 1李文郎 1杨美玲2
作者信息
- 1. 广东省深圳市龙华新区中心医院检验科 518110
- 2. 广东省深圳市龙华新区人民医院 518109
- 折叠
摘要
Abstract
Objective To investigate the influence of heperlipemia and hemolysis specimens on the fluorescence quantitative PCR determination results of low level HBV‐DNA .Methods The serum samples were collected from 100 hepatitis B patients with normal triacylglycerol (TG) (≤1 .46 mmol/L and (4 .0-9 .0) × 103 IU/mL by real time fluorescence quantitative PCR) in our hospital and performed the instant detection .In addition ,100 high TG samples of hepatitis B 5‐item negative and HBV‐DNA non‐detection and 100 whole blood samples of hepatitis B 5‐item nega‐tive and HBV‐DNA non‐detection were collected and performed the simultaneous HBV‐DNA detection of fluores‐cence quantitative PCR on the hyperlipemia and non‐hyperlipemia specimens ,hemolysis specimen and non‐hemolytic serum .And the paired t test was conducted .Results The HBV‐DNA detection levels had statistically significant differences between hyperlipemia and non‐hyperlipemia specimens and between hemolytic serum and non‐hemolytic serum (P<0 .05) .Conclusion The fluorescence quantitative PCR detection results of hyperlipemia and hemolysis specimens of low HBV‐DNA level are reduced compared with those of instant detection for fasting collection speci‐mens .关键词
标本/溶血/高脂血/荧光定量聚合酶链反应Key words
specimen/hemolysis/hyperlipemia/fluorescence quantitative PCR引用本文复制引用
刘小敏,唐恒锋,李文郎,杨美玲..高脂血、溶血标本对荧光定量聚合酶链反应测定低水平HBV-DNA的影响[J].检验医学与临床,2015,(21):3217-3218,3220,3.
