检验医学与临床Issue(22)：3294-3296,3.DOI:10.3969/j.issn.1672-9455.2015.22.002
不同采血管在不同存放条件下对循环酶法同型半胱氨酸测定结果的影响
Influence of different blood collection tubes and different storage conditions on results of homocysteine detection by enzy-matic cycle method
摘要
Abstract
Objective To study the influence of different blood sample collection tubes ,different storage time and different storage temperatures on the results of homocysteine(Hcy) detection by enzymatic cycle assay .Methods The venous blood sample was collected from 32 individuals undergoing the healthy physical examination .The gen‐eral vacuum blood collection tubes ,procoagulant vacuum blood collection tubes ,lithium heparin anticoagulation vacu‐um blood collection tubes and EDTA‐K2 anticoagulation vacuum blood collection tubes were respectively adopted to collect 2 tubes of venous blood for each kind of tube .The same blood tubes from the same person were divided into the room temperature group and the cold storage group respectively ,centrifuged after giving serial number and sto‐ring for 0 .5 h at the room temperature ,Hcy in the room temperature group was detected at once ,while the cold stor‐age group was stored in the fridge;meanwhile 200 μL of serum and plasma were extracted from the general tube and lithium heparin anticoagulation tube and placed into 1 mL EP tube for storage in fridge at 2 -8 ℃ (separation group) .The Hcy levels in the room temperature group and the cold storage group were detected at 3 ,6 ,9 h after cen‐trifugation .After detection ,the general tube and lithium heparin anticoagulation tube in the cold storage group were continuously placed in fridge(non‐separation group) .The Hcy levels in the separation group and the non‐separation group were detected at 24 ,48 h .Results The serum Hcy instant values of 4 kinds of blood collection tube for serum specimen were significantly higher than those for plasma specimen(P<0 .05) .The Hcy levels had no statistically sig‐nificant differences between the placing for 3 ,6 ,9 h by the same collection tube in the same group and the instant val‐ues(P>0 .05) .In the comparison of the Hcy detection results of the same blood collection tube at the same detection time between the room temperature group and the cold storage group ,the detection value at 9 h had statistical differ‐ence(P<0 .05) .The Hcy levels at 48 h under 2-8 ℃ in the general tube serum and lithium heparin anticoagulation tube plasma without timely separating blood cell layers had statistical difference compared with the results detected at 48 h(P<0 .05) .Conclusion Different blood collection tubes ,different storage time and different storage tempera‐tures will affect the detection results of Hcy by enzymatic cycle assay .关键词
同型半胱氨酸/稳定性/采血管/循环酶法Key words
homocysteine/stability/blood collection tubes/enzymatic cycle assay引用本文复制引用
李慧,白利芬,惠玉芬,叶文瑞,雷光星,王兴宁..不同采血管在不同存放条件下对循环酶法同型半胱氨酸测定结果的影响[J].检验医学与临床,2015,(22):3294-3296,3.基金项目
国家高新技术研究发展计划（863计划）子课题（2011AA02A111）。 （）
