检验医学与临床Issue(22)：3307-3308,3311,3.DOI:10.3969/j.issn.1672-9455.2015.22.006
实时荧光定量 PC R 技术诊断人骨肉瘤 M G63细胞系中β-catenin 基因表达的临床分析
Clinical analysis on real time FQ-PCR technique for diagnosingβ-catenin gene expression in human osteosarcoma MG63 cell line
摘要
Abstract
Objective To analyze the real time FQ‐PCR technique for diagnosing β‐catenin gene expression in human osteosarcoma MG63 cell line .Methods Human osteosarcoma cell line MG63 was cultured and grouped .The control group used 10% fetal bovine serum and the culture medium containing high glucose DEME penicillin and streptomycin .On this basis the stimulation group was cultured by using 100ng/mL wnt3a cell culture .The cells in 3 holes were collected on 0 ,1 ,2 ,3 ,4 d respectively and the cells number was counted by adopting the cell digestion way .FQ‐PCR was adopted to detect theβ‐catenin gene expression in human osteosarcoma cell line MG63 .Results On 0 ,1 d of the cell growth ,the MG cells number had no statistical difference between the two groups ;while which on 2 ,3 ,4 d had statistically significant differences between the two groups(P<0 .05) .Theβ‐catenin gene expression showed a non‐positive correlation with the stimulation concentration of wnt3 protein ,in various concentrations ,theβ‐catenin gene expression stimulated by 100 ng/mL wnt3a protein was highest ,the differences in theβ‐catenin gene ex‐pression had no statistical difference among other concentrations(P>0 .05) .Theβ‐catenin gene expression stimulated by 100 ng/mL wnt3a protein for 6 h culture reached highest ,moreover before 6 h ,theβ‐catenin gene expression was increased with time lapse .Theβ‐catenin gene expression after 6 h had no statistical difference(P>0 .05) .Conclusion FQ‐PCR can reflect theβ‐catenin gene expression in human osteosarcoma MG63 cells .关键词
人骨肉瘤细胞/β-catenin/实时荧光定量PCR技术Key words
human osteosarcoma/β-catenin/FQ-PCR引用本文复制引用
林国叶,谢志平..实时荧光定量 PC R 技术诊断人骨肉瘤 M G63细胞系中β-catenin 基因表达的临床分析[J].检验医学与临床,2015,(22):3307-3308,3311,3.基金项目
福建省自然科学基金资助项目（2012J01410）。 （）
