检验医学与临床Issue(22)：3342-3345,4.DOI:10.3969/j.issn.1672-9455.2015.22.019
Ⅱ型前胶原羧基末端前肽对骨性关节炎早期诊断价值的实验研究
Experimental research of early diagnostic value of C-terminal propeptide of type Ⅱ procollagen for osteoarthritis
李明宇 1安宇 1侯利民1
作者信息
- 1. 哈尔滨医科大学附属第一医院急诊外科，哈尔滨 150001
- 折叠
摘要
Abstract
Objective To study the diagnostic value of C‐terminal propeptide of type Ⅱ procollagen(PⅡCP) in early osteoarthritis(OA) through monitoring the changes of PⅡCP in the synovial fluid and serum of the OA mod‐el rat .Methods Wistar rats were randomly divided into the OA model group and the sham operation group .The rat knee‐joint OA model was induced by the improved Hulth surgery method .The synovial fluid ,serum and joint speci‐mens were weekly taken from 6 rats in each group at 1-5 weeks after modeling ,the serum and synovial fluid PⅡCP levels were measured by ELISA .The general observation score and histopathological score of articular cartilage were obtained .The differences at various time points were compared between the two groups and the change rule of PⅡCP levels and cartilage scores with time was compared .The Pearson correlation coefficient and linear regression analysis were adopted to test the correlation between serum and synovial fluid PⅡCP levels .Results The histologic manifes‐tations of cartilage at postoperative 1 week in the OA model group were similar to those in the sham operation group , and the obvious pathologic change gradually started to appear at postoperative 2 weeks .The cartilage histology of the sham operation group had no obvious change .Compared with the sham operation group ,the serum and synovial fluid PⅡCP levels in the OA model group started to obviously increase at postoperative 1 week ,and the difference was gradually increased with time lapse;the humoral PⅡCP levels of the sham operation group maintained a lower level from beginning to end;the serum PⅡCP level had a good correlation with the synovial fluid PⅡCP level .Conclusion In rat OA model ,the serum and synovial fluid P Ⅱ CP levels are increased before the cartilage appears obvious pathological change ,and the P Ⅱ CP levels are consistent with the OA pathological change ,so the serum and synovial fluid P Ⅱ CP levels could be used as the reference indicators of OA early diagnosis .关键词
骨性关节炎/Ⅱ型前胶原羧基末端前肽/软骨/诊断Key words
osteoarthritis/C-terminal propeptide of type Ⅱ procollagen/cartilage/diagnosis引用本文复制引用
李明宇,安宇,侯利民..Ⅱ型前胶原羧基末端前肽对骨性关节炎早期诊断价值的实验研究[J].检验医学与临床,2015,(22):3342-3345,4.
