检验医学与临床Issue(23)：3522-3524,3527,4.DOI:10.3969/j.issn.1672-9455.2015.23.029
不同温度热疗联合多西紫杉醇对人乳腺癌细胞株增殖的影响
Synergistic effect of docetaxel plus hyperthermia of different temperatures on the proliferation of human breast carcino-ma cells
崔宝林 1宁晓娥2
作者信息
- 1. 陕西省宝鸡市妇幼保健院 乳腺科 721000
- 2. 陕西省宝鸡市妇幼保健院 计划生育科 721000
- 折叠
摘要
Abstract
Objective To explore the synergistic effect of docetaxel plus mild hyperthermia on the proliferation of human breast carcinoma cells and its mechanism .Methods M T T assay was used to test the inhibitory effect of do‐cetaxel on the proliferation of human breast carcinoma MCF‐7 cells ,and the effective concentration of docetaxel was screened .The human breast carcinoma MCF‐7 cells cultured in vitro were divided into blank control group ,docetaxel group and docetaxel plus hyperthermia group .The docetaxel plus hyperthermia group was divided into 5 subgroups (39 .0 ℃ group ,39 .5 ℃ group ,40 .0 ℃ group ,40 .5 ℃ group and 41 .0 ℃ group) ,according to the different temper‐atures of hyperthermia .Flow cytometry was used to detect the cell apoptosis and the change of cell growth cycle in every group after intervention .Results The apoptosis rate of cells increased with the temperature of hyperthermia . 41 .0 ℃ group had the highest apoptosis rate of cells [(38 .90 ± 6 .88)% ] ,which was significantly higher than do‐cetaxel group and other subgroups of docetaxel plus hyperthermia group .The activity of c‐Jun amino‐terminal kinase reached maximum when the hyperthermia temperature was 41 .0 ℃ ,while the activities of ERK1/2 and p‐p38 reached maximum when the hyperthermia temperature was 40 .0 ℃ ,which was significantly different from other subgroups of docetaxel plus hyperthermia group(P< 0 .05) .The proportions of cells at G2 /M phase in the subgroups of docetaxel plus hyperthermia group were significantly higher than docetaxel group .The inhibitory effect of docetaxel plus hyper‐thermia on the MCF‐7 cell cycle was stronger than docetaxel alone .The proportion of cells at G2 /M phase increased with the hyperthermia temperature ,with statistical difference(P< 0 .05) .Compared with docetaxel group ,the expres‐sion level of mitogen activated protein kinase pathway protein in docetaxel plus hyperthermia group was significantly higher ,the expression level of Bcl‐2 significantly reduced ,and the expression level of Bax slightly increased .Conclu‐sion Docetaxel plus hyperthermia could inhibit the proliferation of human breast carcinoma cells and induce the ap‐optosis ,which showed a synergistic antitumor effect .The mechanism might relate with the activation of extracellular regulated protein kinase ,c‐Jun amino‐terminal kinase and p38 .关键词
热疗/多西紫杉醇/乳腺癌Key words
hyperthermia/docetaxel/breast carcinoma引用本文复制引用
崔宝林,宁晓娥..不同温度热疗联合多西紫杉醇对人乳腺癌细胞株增殖的影响[J].检验医学与临床,2015,(23):3522-3524,3527,4.
