林产化学与工业Issue(5)：5-9,5.DOI:10.3969/j.issn.0253-2417.2015.05.002
酶水解过程中纤维素聚合度和结晶度的变化
Changes of Cellulose Polymerization and Crystallinity Degrees during Enzymatic Hydrolysis
摘要
Abstract
Complex cellulase named Celluclast 1. 5L and endo-cellulase named Novozym 476 were applied to hydrolyze the bleached softwood fiber. In the present study, the influences of these two kinds of cellulases on the degree of polymerization ( DP) and crystallinity of cellulose were investigated. The results indicated that the DP of cellulose decreased gradually with the increase of the enzyme dosage after hydrolyzed by both cellulases. While the dosage of Celluclast 1. 5 L was 3. 0 FPU/g, the yield of cellulose was 94. 34%. The DP of cellulose decreased to 862 and decreased by 25. 95% compared with the control sample (1 164). While the Novozym 476 was in the dosage of 50. 0 U/g, the cellulose yield was 94. 93%. The DP of cellulose decreased to 711 and decreased by 38. 92% compared with the control sample. This showed that in the circumstances of same yield, the cellulose hydrolyzed by Novozym 476 had a lower DP compared with the cellulose hydrolyzed by Celluclast 1. 5 L. It meant that endoglucanase was the main factor which led to the decline of DP. With the increase of enzyme dosage of Celluclast 1. 5 L, cellulose crystallinity took the changes of M-type, in other words, increased first and then reduced, and finally increased and reduced again. With the increase of enzyme dosage of Novozym 476, the cellulose crystallinity decreased first, and then increased and decreased again.关键词
纤维素酶/漂白针叶木纤维/水解/聚合度/结晶度Key words
cellulase/bleached softwood fiber/hydrolysis/degree of polymerization/crystallinity degree分类
化学化工引用本文复制引用
杜敏,李新平,陈立红,王志杰,李鸿魁..酶水解过程中纤维素聚合度和结晶度的变化[J].林产化学与工业,2015,(5):5-9,5.基金项目
国家自然科学基金面上项目(31370578) （31370578）
陕西省科技厅省级重点实验室项目(2011HBSZS014) （2011HBSZS014）
陕西科技大学科研启动基金项目(BJ13-21) （BJ13-21）
