临床神经病学杂志2015，Vol.28Issue(5)：371-374,4.
经皮三叉神经电刺激对匹罗卡品诱导的癫痫大鼠的行为及学习记忆能力的影响
Effects of percutaneous trigeminal nerve electrical stimulation on behavior and the ability of learning and memory of epileptic rats induced by Pilocarpine
摘要
Abstract
Objective To observe the effects of percutaneous trigeminal nerve electrical stimulation on behavior and the ability of learning and memory of epileptic rats induced by Pilocarpine.Methods Thirty healthy male rats were randomly divided into normal control group, epilepsy model group and trigeminal nerve stimulation group ( TNS group) .Pilocarpine was used to inducestatus epileptics ( SE) in rats.TNS group rats were treated with percutaneous trigeminal nerve stimulation consecutive for 4 weeks.At 2 weeks after SE model established, video monitor were employed to record the frequency of spontaneous recurrence seizures ( SRS) , the intensity and duration of seizures. At 4 weeks after SE model established, rats in each group were taken Morris water maze test.The incubation period of rats finding underwater platform in the acquired training was recorded.The percentage of time and distance of rats searching target quadrant and the number of times through the platform were also recorded.Results Rats in control group didn''t show abnormal behavior throughout this experiment.During the process of animal model making, a rat died in epilepsy model group while 2 in TNS group.During-EEG monitoring, 2 rats in epilepsy model group died. Compared with epilepsy model group, the frequency of spontaneous recurrence seizure and the intensity of seizure significantly decreased, the average time of seizure significantly shortened in TNS group ( P<0.05 -0.01 ) in 2 weeks.In acquired training experiment, with the number of training increased, the average escape latent of experimental animals gradually reduced, the escape latent of rats in epilepsy model group were significantly prolonged compared with control group rats at each time-point ( all P<0.01 ) , while the average escape latency was significantly reduced in TNS group rats than epilepsy model group (P<0.05-0.01).Compared to control group, the percentage of time search and the percentage of navigation distance in the target quadrant and the number of cross platform were significantly decreased in epilepsy model group and TNS group rats (all P<0.01).Compared to epilepsy model group, the percentage of time search and the percentage of navigation distance in the target quadrant within 60s were significantly higher and more cross platform numbers in TNS group ( P<0.05-0.01) .Conclusion In experimental epilepsy, percutaneous trigeminal nerve stimulation can reduce spontaneous recurrence seizures of the temporal lobe epilepsy rat and protect the ability of learning and memory.关键词
癫痫/匹罗卡品/经皮三叉神经电刺激/Morris水迷宫试验Key words
epilepsy/Pilocarpine/percutaneous trigeminal nerve electrical stimulation/Morris water maze test分类
医药卫生引用本文复制引用
王倩倩,刘益民,钟平,张雷,王玉..经皮三叉神经电刺激对匹罗卡品诱导的癫痫大鼠的行为及学习记忆能力的影响[J].临床神经病学杂志,2015,28(5):371-374,4.基金项目
国家自然科学基金资助项目(30970997,81271444) （30970997,81271444）
安徽省战略性新兴产业科技攻关项目(11010402168) （11010402168）
