临床神经外科杂志Issue(5)：341-345,5.DOI:10.3969/j.issn.1672-7770.2015.05.006
Snail 介导 GOLPH3对人脑胶质瘤细胞迁移和侵袭作用的研究
GOLPH3 promotes glioblastoma cell migration and invasion via Snail
摘要
Abstract
Objective To study the role and mechanism of Golgi phosphoprotein 3(GOLPH3) in glioma cell migration and invasion .Methods The effect of GOLPH3 on the expression of YB1,snail and MMP2 was examined by Western blotting and RT-PCR respectively .After down-regulation of snail, the protein level of YB1 were analyzed by western blotting ,cell migration and invasion ability were examined by wound healing and invasion assay respectively .After GOLPH3 over-expression with or without Snail down-regulation,GOLPH3,Snail and YB1 protein expression levels in U251 glioma cells were determined by western blotting , and the ability of cell migration and invasion were examined by wound healing and invasion assay respectively .Results Down-regulation of GOLPH3 decreased the protein and mRNA levels of YB 1 and mRNA levels of MMP2.Down-regulation of GOLPH3 decreased the protein level of Snailwhile over-expression of GOLPH3 showed contrary effect . Down-regulation of Snail decreased the level of YB 1 and inhibited the migration and invasion of U 251 cells.Over-expression of GOLPH3 inducing U251 cell migration and invasion were blocked by Snail down-regulation.Conclusion Down-regulation of GOLPH3 inhibited glioma U251 cell migration and invasion through regulating Snail and YB 1 expression .关键词
脑胶质瘤/迁移/侵袭/GOLPH3/Snail/YB1Key words
glioma/migration/invasion/GOLPH3/Snail/YB1分类
医药卫生引用本文复制引用
赵伟,张旭,陆冬,张昊,石琼,周秀萍,于如同..Snail 介导 GOLPH3对人脑胶质瘤细胞迁移和侵袭作用的研究[J].临床神经外科杂志,2015,(5):341-345,5.基金项目
国家自然科学基金项目 （）
