河南科技大学学报（医学版）2015，Vol.33Issue(3)：161-163,3.
HPLC法测定山茱萸果核中5种有机酸的含量
Determination of Five Kinds of Organic Acids in Cornus Officinalis Kernels by High Performance Liquid Chromatography
摘要
Abstract
Objective To establish a quantitative HPLC method for determining five kinds of organic acids(oxalic acid, tartaric acid, DL-malic acid, citric acid and gallic acid) in cornus officinalis kernels. Methods A Kromasil C18 column(250 mm × 4. 6 mm i. d. , 5 μmm) was used with 3% CH3OH-0. 01 mol·L-1 KH2PO4(pH 3. 0)as the mobile phase at a flow rate of 0. 5 mL·min-1. The column temperature was 25℃ and the UV detection wavelength was 210 nm. Results The five organic acids were separated in 32 min. The average recoveries of the method were 89. 6% ~102. 0% with the relative standard deviation of 0. 29% ~1. 41%(n=6). Conclusion The method is simple, accurate, reproducible and practical for the detection of organic acids in cornus officinalis kernels.关键词
HPLC/山茱萸/果核/有机酸Key words
high performance liguid chromatography/Cornus officinalis/kernel/organic acids分类
医药卫生引用本文复制引用
杨晖,和素娜,李杰,段冷昕,李艳,王建刚..HPLC法测定山茱萸果核中5种有机酸的含量[J].河南科技大学学报（医学版）,2015,33(3):161-163,3.基金项目
国家自然科学基金(U1204826)河南科技大学实验技术开发基金资助项目( SY1415034/SY1415036) （U1204826）
