泸州医学院学报Issue(2)：124-128,5.
GDNF基因修饰的BMSCs向神经元样细胞的分化及神经营养因子的表达*
Differentiation of GDNF gene-modified bone marrow mesenchymal stem cells into neuron-like cells and expression of neurotrophic factors
摘要
Abstract
Objective: To investigate the differentiation of GDNF gene-modified bone marrow mesenchymal stem cells into neuron-like cells and the expression of neurotrophic factors. Methods: GDNF recombinant aden-ovirus vector and blank virus plasmid (BVP) were used to infect rat BMSCs, to obtain GDNF-BMSCs and BVP-BMSCs. Immunofluorescence staining was used to observe the number of neuron specific marker,the mircotubule-associated protein 2 (MAP2)-positive cells.RT-PCR technique was used to examine the expression levels of MAP2 and GAP-43 mRNA in cell pellets. ELISA assay was employed to detect the expression levels of GDNF and NGF protein in supernatant. Results: In the BVP/BMSCs group, no MAP2-positive staining was observed and MAP2 and GAP-43 mRNA expression were nearly undetectable. In the GDNF/BMSCs group, MAP2-positive rate was (42.21±4.79)% and the mRNA of MAP2 and GAP-43 was observed.The expression of GDNF and NGF proteins was higher in the GDNF/BMSCs group than in the BVP/BMSCs group (P<0.05). Conclusion:GDNF has the effect of promoting the differentiation of BMSCs to neuron-like cells, and its effect may be correlated with the up-regu-lation of expression of GAP-43, GDNF and NGF.关键词
GDNF/神经元样细胞/骨髓间充质干细胞/NGFKey words
GDNF/Neuron-like cells/BMSCs/NGF分类
医药卫生引用本文复制引用
杜杰,高小青,郭侃,邓莉,常能彬..GDNF基因修饰的BMSCs向神经元样细胞的分化及神经营养因子的表达*[J].泸州医学院学报,2013,(2):124-128,5.基金项目
四川省卫生厅项目 （）
