泸州医学院学报Issue(3)：211-215,5.DOI:10.3969/j.issn.1000-2669.2013.03.003
3H－TdR对骨髓间充质干细胞代谢的量效关系研究*
Effect of dosage of 3H-TdR on biological characteristics of mesenchymal stem cells
摘要
Abstract
Objective:To study the cellular toxicity of 3H-TdR in labeling mesenchymal stem cells (MSCs). Methods: Different dosage of 3H-TdR was added to the culture bottles. The growth curves of BMSC in different concentrations were made, the apoptosis of cell was observed by Hochest stain and the cell cycle was observed with flow cytometer. The cell surface marks of MSCs( CD29,CD166,CD44,CD45) were detected by flow cytometer and the influence on SOD or MDA level was observed. Results: The researches showed that MSCs growth was suppressed at 4μCi/2ml concentration of 3H-TdR. High concentration of 3H-TdR caused MSCs to be arrested at the S and G2-M stage; MSCs showed obvious apoptosis when the concentration of 3H-TdR exceeded 3μCi/2ml;SOD decreased,MDA increased and their changes were in proportion to the alteration of concentration of 3H-TdR. The proliferation ability of successive progeniture was not affected by 3H-TdR when its concentration didn't exceed 1μCi/2ml. Conclusion: Higher concentration of 3H-TdR can arrest cell cycle of stem cell,affect its growth and tracer function,but concentration lower than 1μCi/2ml has no effect on biological characteristic of stem cell.Therefore it is safe for tracing study.关键词
3H-TdR/毒性/MSCs/细胞表面标志物/细胞周期Key words
3H-TdR/Toxicity/MSCs/Cell surface marks/Cell cycle分类
医药卫生引用本文复制引用
李经伦,吕英姿,何晓英,冯善伟,张成..3H－TdR对骨髓间充质干细胞代谢的量效关系研究*[J].泸州医学院学报,2013,(3):211-215,5.基金项目
国家自然基金资助项目 （）
