泸州医学院学报Issue(3)：220-222,3.DOI:10.3969/j.issn.1000-2669.2013.03.005
shRNA对小鼠Lewis细胞C-erbB-2基因表达的影响*
Inhibitive effect of shRNA on expression of C-erbB-2 gene in Lewis cells
摘要
Abstract
Objective: To investigate the inhibitive effect of shRNA on the expression of C-erbB-2 gene in Lewis cells. Methods: pGPU6/RFP/Neo -shNC plasmid and 4 pGPU6/RFP/Neo -erbB -2 plasmids were synthesized. After pGPU6/RFP/Neo-shNC plasmid was transfected into mouse Lewis cells with the ratio of DNA to Lipofectamine 2000 at 1∶2,1∶2.5,and 1∶3;fluorescence microscope was used to judge the best transfection effect. The experiment was divided into the following groups:pGPU6/RFP/Neo-erbB-2-mus-347group,pGPU6/RFP/Neo-erbB-2-mus-1944 group, pGPU6/RFP/Neo-erbB-2-mus- 2310 group, pGPU6/RFP/Neo-erbB-2-mus-3669 group,pGPU6/RFP/Neo-shNC group and control group.After transfection, C-erbB-2 mRNA and protein was tested by RT-PCR and western blotting,respectively. Results: The transfection effect was the best when DNA∶Lipofectamine 2000 was 1∶2.5. All the 4 pGPU6/RFP/Neo-erbB-2 plasmids could decrease the level of C-erbB-2 mRNA and protein,and pGPU6/RFP/Neo-erbB-2-mus-3669 had the best interference effect. Conclusion:pGPU6/RFP/Neo-erbB-2-mus-3669 can inhibit the expression of C-erbB-2 gene in Lewis cells.关键词
C-erbB-2/shRNA/Lewis细胞Key words
C-erbB-2/shRNA/Lewis cells分类
医药卫生引用本文复制引用
曹新梅,张岱权,王栩,郑春燕..shRNA对小鼠Lewis细胞C-erbB-2基因表达的影响*[J].泸州医学院学报,2013,(3):220-222,3.基金项目
四川省卫生厅资助项目 （）
