泸州医学院学报Issue(3)：223-227,5.DOI:10.3969/j.issn.1000-2669.2013.03.006
脑出血PAR-1、HIF-1α与细胞凋亡关系实验研究*
Experimental study of proteinase activated receptor-1 and hypoxia inducible factor-1αin rats after cerebral hemorrhage
摘要
Abstract
Objective: To observe the expression of PAR-1and HIF-1α neuronal apoptosis in rats after cerebral hemorrhage. Methods: 90 SD rats were randomly divided into normal control group (n=30), sham operation group (n=30),and ICH group (n=30). Autologous blood was injected into the caudate nucleus of rat to produce cerebral hemorrhage model by using stereotaxie technique. Decapitation was taken at 6hr, 1d, 2d, 3d, 5d and 7d after the model was produced. PAR-1 positive cell, HIF-1α positive cell, and TUNEL positive cell,were detected by immunohisto-chemistry,and VEGF in intracerebral hemorrhage group.Results: There was only a few PAR-1,HIF-1α expression in normal control group,sham operation group and difficultly observated apoptotic cell (TUNEL positive cell). PAR-1 positive cell and TUNEL cell increased in intracerebral hemorrhage group at 6hr, PAR-1 positive cell and TUNEL cell reached a high level at 3d, PAR-1 positive cell descended to normal level at 7d, but TUNEL cell reached a higher level at 7d. HIF-1α was not expressed obviously in intracerebral hemorrhage group at 6h, increased obviously at 1d, reached a high level at 3d, but expressed a higher level at 7d, had statistical significance to normal control group,compare sham operation group (P<0.05). VEGF was expressed obviously in intracerebral hemorrhage group at 6hr, reached a high level at 3d, but a higher level at 7d, statistically different from normal control group,sham operation group (P<0.05).Conclusion:PAR-1, apoptotic cell in the brian hematoma perienchyma markedly increased after ICH;HIF-1αpositive cell,VEGF contents obviously increased.关键词
脑出血/PAR-1/HIF-1α/VEGF/细胞凋亡Key words
Intracerebral hemorrhage/Proteinase activated receptor-1/Hypoxia inducible factor-1α/Vascular endothelial growth factor/Apoptotic cell分类
医药卫生引用本文复制引用
张德绸,刘大勇,白雪,李小林,江燕..脑出血PAR-1、HIF-1α与细胞凋亡关系实验研究*[J].泸州医学院学报,2013,(3):223-227,5.基金项目
四川省卫生厅资助项目 （）
