泸州医学院学报2015，Vol.38Issue(5)：437-440,4.DOI:10.3969/j.issn.1000-2669.2015.05.001
BIOMED-2系统应用于B细胞非霍奇金淋巴瘤抗原受体基因重排检测的研究
Detection of B-cell Non-Hodgkin lymphoma antigen rearrangement using BIOMED-2 system in paraffin-embedded specimens
黄娟 1刘浩 2曾凡才 3陈明 1刘萍 1莫冬阳 1李鑫1
作者信息
- 1. 四川医科大学 附属第一医院病理科,四川泸州 646000
- 2. 四川医科大学 附属中医院胸外科,四川泸州 646000
- 3. 四川医科大学 生物化学教研室,四川泸州 646000
- 折叠
摘要
Abstract
Objective: To evaluate the BIOMED-2 system in detecting the monoclonality of antigen receptor gene rearrangement in B-cell Non-Hodgkin lymphoma. Methods: Genomic DNA was extracted from paraffin-embedded specimens of 40 cases of B cell Non-Hodgkin lymphoma and 10 cases of proliferative diseases of lymph nodes, and clonality of antigen receptor gene rearrangement was assessed using the 9 groups of total 61 primers of BIOMED-2 system. Results: 94% (47/50) of specimens yielded PCR products over 300 bp using DNA quality control primers. The sensitivity and specificity of BIOMED-2 system in the detection of monoclona-lity of antigen receptor gene rearrangement were both 100%. The rate of detection of monoclonality using primes for IgH-A,IgH-B,IgH-C,IgH-D,IgH-E,IgK-A,IgK-B,IgL was 45%(18/40),27.5%(11/40),87.5%(35/40), 55%(22/40),47.5%(19/40),80%(32/40),50%(20/40) and 22.5%(9/40)respectively. When combining IgH-C and IgK-A primers, all cases of antigen receptor gene monoclonality rearrangement could be detected. Conclusion:BIOMED-2 system is a highly sensitive and specific method in detecting the monoclonality of antigen receptor gene rearrangement of B cell Non-Hodgkin lymphoma in routine paraffin embedded specimens.关键词
BIOMED-2系统/非霍奇金淋巴瘤/B细胞/抗原受体/基因重排Key words
BIOMED-2 system/Non-Hodgkin lymphoma/B-cell/antigen receptor/Gene rearrangement分类
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黄娟,刘浩,曾凡才,陈明,刘萍,莫冬阳,李鑫..BIOMED-2系统应用于B细胞非霍奇金淋巴瘤抗原受体基因重排检测的研究[J].泸州医学院学报,2015,38(5):437-440,4.
