泸州医学院学报2015，Vol.38Issue(5)：461-464,4.DOI:10.3969/j.issn.1000-2669.2015.05.007
CAG治疗方案药物对人Jurkat细胞的增殖抑制及诱导凋亡作用
Effect of CAG regimen on the inhibition of proliferation and enhancement of apoptosis in Jurkat cells
摘要
Abstract
Objective: To investigate the inhibition of proliferation and enhancement of apoptosis in Jurkat cells induced by CAG regimen. Methods: Jurkat cells and bone marrow cells of patients with iron deficiency anemia were treated by CAG regimen (G-CSF,Ara-C and ACR), and the proliferation inhibition of the cell was detected by CCK-8 assay. The apoptosis rate was detected by flow cytometry in Annexin V FLUOS Staining Kit. Results: The inhibitory proliferation rate of CAG regimen in the Jurkat cells by 48 h was 93.67%, which was significantly higher than that of PBS control group of Jurkat cells. The inhibitory proliferation rate of CAG regimen in the Jurkat cells by 48 h was 91.92%. Compared with CAG regimen on the patients with iron deficiency anemia,the result is statistically significant (P < 0.05). The inhibitory proliferation rate of CAG regimen in the patients with iron deficiency anemia by 48 h was 7.16%, and the difference was not significant between CAG and PBS in patients with iron deficiency anemia. After 12 h treatmemnt by CAG regimen, the early apoptosis rate of Jurkat cells was 46.12%, and the late apoptosis rate was 5.79%. After 24 h treatment by CAG regimen, the early apoptosis rate of cells was 55.21%, and the late apoptosis rate was 28.31%. After the treatmemnt by PBS, the apoptosis rate of Jurkat cells was 0%. The result is statistically significant (P< 0.05). Conclusion: The inhibition of proliferation in bone marrow cells treated by the CAG regimen was not obvious. CAG regimen can significantly inhibit the proliferation of Jurkat cells and kill Jurkat cells. CAG regimen can also induce early and late apoptosis, which suggest that apoptosis play a decisive role in the death of Jurkat cells, and the majority of cells be cleared by the apoptosis.关键词
CAG治疗方案/Jurkat细胞株/增殖抑制/凋亡Key words
CAG regimen/Jurkat cells/Proliferation inhibition/Apoptosis分类
医药卫生引用本文复制引用
邓禹,李金遥,刘云垚,杜竺蔓,段宏基,洪静,马明义,赵矫..CAG治疗方案药物对人Jurkat细胞的增殖抑制及诱导凋亡作用[J].泸州医学院学报,2015,38(5):461-464,4.基金项目
四川省大学生创新训练项目(No. 201510632080) （No. 201510632080）
