国际眼科杂志Issue(11)：1857-1861,5.DOI:10.3980/j.issn.1672-5123.2015.11.05
豚鼠早期实验性近视眼视网膜色素上皮细胞 bFGF表达变化的研究
Altered bFGF expression in retinal pigment epithelial cells from guinea pig model with early stage of experimentally-induced myopia
摘要
Abstract
Abstract•AlM:To determine the expression of basic fibroblast growth factor ( bFGF) in retinal pigment epithelial ( RPE) cells from anterior and posterior part of guinea pig eyes with lens-induced myopia ( LlM) .•METHODS:Thirty two-week-aged guinea pigs were randomly divided into three groups, group A, group B and group C. Another five guinea pigs ( 10 eyes ) were chosen as control group without any treatment. Concave lenses were worn by the 30 guinea pigs on either side of their eyes. After 6, 15 and 30d, the lenses were removed and optometry and axial length were used to make sure the myopia had formed. The retinal pigment epithelial cells of each group were cultured and passaged in vitro using enzymatic digestion method. The cultured cells at 3~6 generation was detect by immunocytolchemistory, Real- Time PCR and Western blotting to detect the expression of bFGF.•RESULTS:The expression of bFGF was located in the cytoplasm and nucleus. The results of immunocytolchemistory, Real-Time PCR and Western blotting showed that the expression of bFGF were detected at anterior and posterior part of eyes from experiment group and control group, the expression in experiment group at both part was significantly lower than those in control group ( P<0. 05 ). The positive percentage of expression of bFGF in experiment group decreased with the induced time ( P < 0. 05 ). The expression of bFGF from anterior part of both experiment group and control group was as the same as that from posterior part of themselves (P>0. 05).•CONCLUSlON:The expression of bFGF from anterior and posterior part of experiment group was lower than those of control group.关键词
豚鼠/视网膜色素上皮细胞/碱性成纤维细胞生长因子/免疫细胞化学/实时荧光定量PCR/Western-blot蛋白印迹法Key words
guinea pig/retinal pigment epithelial cells/basic fibroblast growth factor/immunocytochemistry/Real - Time PCR Analysis/Western blotting引用本文复制引用
陈博宇,王超英,陈维毅,刘迎庆,郝岚..豚鼠早期实验性近视眼视网膜色素上皮细胞 bFGF表达变化的研究[J].国际眼科杂志,2015,(11):1857-1861,5.基金项目
河北省自然科学基金资助项目(No. H2012505009)@@@@Natural Scinece Foundation of Hebei Province (No. H2012505009) （No. H2012505009）
