中南医学科学杂志Issue(6)：614-617,684,5.DOI:10.15972/j.cnki.43-1509/r.2015.06.004
肺炎嗜衣原体包涵体膜蛋白Cpn0147诱导单核细胞分泌TNF-α和IL-8
Chlamydophila pneumonia-Derived Inclusion Membrane Protein Cpn0147 Induces Monocytes Secretion of TNF-αand IL-8
摘要
Abstract
Objective To investigate the molecular mechanism of the inclusion membrane protein Cpn0147 on the se-cretion of cytokines in monocytes. Methods Cultured THP-1 cells were stimulated with different concentration of endotox-in-free recombinant inclusion protein (1. 0,10. 0,30. 0 and 50. 0μg/mL) for 0~48 h. Secretion of TNF-αand IL-8 were de-tected by ELISA,expression of the mRNA was measured by real-time PCR. In addition,cells were preincubated with Toll-like receptor 2(TLR2) or TLR4 neutralizing antibody,or transfected with siRNA for TLR2 and TLR4,secretion of TNF-αand IL-8 were detected by ELISA. Results Cpn0147 could induce THP-1 cells the secretion of TNF-αand IL-8,and the expres-sion of mRNA. The production of TNF-αand IL-8 varies with time intervals,the ELISA results indicated secretion of cytokines appeared after 2~6 h of stimulation,peaked at 12 h and then decreased. Pre-incubation of TLR2 or TLR4 neutralizing anti-body significantly abrogate Cpn0147-induced cytokines production,similar results was also obtained by RNA interference of TLR2 and TLR4. Conclusion Cpn0147 can induce TNF-αand IL-8 secretion via TLR2 and TLR4.关键词
肺炎嗜衣原体/Toll样受体2/Toll样受体4/肿瘤坏死因子-α/白介素-8Key words
Chlamydophila pneumonia/Toll-like receptor 2/TLR4/TNF-α/IL-8分类
医药卫生引用本文复制引用
吴华,卿文衡,刘军,张黎黎..肺炎嗜衣原体包涵体膜蛋白Cpn0147诱导单核细胞分泌TNF-α和IL-8[J].中南医学科学杂志,2015,(6):614-617,684,5.基金项目
湖南省卫生厅科技计划项目(B2013-132) （B2013-132）
