南京林业大学学报（自然科学版）2015，Vol.39Issue(5)：21-26,6.DOI:10.3969/j.issn.1000-2006.2015.05.004
绛红褐链霉菌的根际定殖能力动态监测
Dynamic detection for colonization of Streptomyces purpeofuscus in Juglans sigllata rhizosphere
摘要
Abstract
Fusarium solani is an important pathogen on Juglans sigllata causing root rot, which can be strongly antago?nistic by the strain Streptomyces purpeofuscus YSSPG3. In order to monitor the colonization dynamics of S. purpeofuscus on rhizosphere soil and the dynamic variation of F. solani, the same infectious indices of J. sigllata were studied as the re?search objects. Based on inoculating S. purpeofuscus to the pathogenetic plants,the dynamic changes of S. purpeofuscus and F. solaniin rhizosphere soil was investigated by real?time fluorescence quantitative PCR, and the dynamic coloniza?tion detection system of S. purpeofuscus and the dynamic change system of fungus were established respectively. The re?sults showed that the strain S.purpeofuscus YSSPG3 could multiplied rapidly in a short time after inoculation, the strain peaked on the 20th day and then declined until the 40th day, finally, it kept stable. On the 60th day, the relatively ex?pression quantity of F. solani roped nearly 11 500%, which showed significantly biocontrol effects on F. solani. The study provided a reliable technical support on the real?time monitoring of plant diseases caused by F. solani, it also layed an important theoretical foundation on the colonization detection of S. purpeofuscus in rhizosphere.关键词
绛红褐链霉菌/腐皮镰刀菌/实时荧光定量PCR/定殖能力/生物防治Key words
Streptomyces purpeofuscus/Fusarium solani/real?time fluorescence quantitative PCR/colonization ability/biological control分类
农业科技引用本文复制引用
谯天敏,郑磊,张静,朱天辉,陈慧..绛红褐链霉菌的根际定殖能力动态监测[J].南京林业大学学报（自然科学版）,2015,39(5):21-26,6.基金项目
国家科技基础条件平台建设项目(2005DK21207-13) （2005DK21207-13）
