南京林业大学学报（自然科学版）Issue(6)：7-11,5.DOI:10.3969/j.issn.1000-2006.2015.06.002
DNA条形码试剂盒检测技术在墨天牛鉴定中的应用
The application of DNA barcoding identification kit technology to Monochamus Dejean
摘要
Abstract
In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene ( mitochondrial cyto⁃chrome oxidase subunit I) . Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and up⁃loaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification.关键词
墨天牛属/COⅠ基因/DNA条形码试剂盒/标记性碱基Key words
Monochamus/COⅠ gene/DNA barcoding identification kit technology/specific site/quarantine分类
农业科技引用本文复制引用
陈梦义,徐梅,钱路,安榆林,郝德君..DNA条形码试剂盒检测技术在墨天牛鉴定中的应用[J].南京林业大学学报（自然科学版）,2015,(6):7-11,5.基金项目
“十二五”国家科技支撑计划（2012BAK11B03）；国家自然科学基金项目（31170606）；江苏高校优势学科建设工程资助项目 （）
