南京林业大学学报（自然科学版）Issue(6)：40-44,5.DOI:10.3969/j.issn.1000-2006.2015.06.008
胡杨未减数2n花粉形成的细胞学机制
Cytological mechanism of unreduced 2n pollen formation in Populus euphratica
摘要
Abstract
In order to analyze abnormal behaviors of chromosomes during meiosis and abnormal organization of microtu⁃bule during meiosis and cytokinesis, and find a new method to illustrate unreduced 2n pollen formation, indirect immu⁃nofluorescence microscopy with DAPI staining was conducted to observe the microtubule cytoskeleton pattern and chro⁃mosomes behavior during meiosis and cytokinesis in Populus euphratica. The results indicated that: ① During the pro⁃phase stage of meiosis, the paired homologous chromosomes combined, loosely, with univalent at diakinesis. Parallel spindle, tripolar spindle and fused spindle could also been found, at metaphase Ⅱ Lagging chromosomes could be ob⁃served at anaphaseⅠand anaphaseⅡ. ②Positive correlations between frequency of 2n pollen and rate of tripolar spindle and fused spindle were found, which suggested that tripolar spindle and fused spindle could result in FDR-typed 2n pol⁃len formation. ③Prematurely cytokinesis was observed at prophaseⅡ, which resulted in SDR-typed 2n pollen formation in Populus euphratica. ④ Due to lack of spindle microtubule in one daughter cell at anaphaseⅡ, the chromatids do not migrated to the poles instead of retaining in the daughter cell and forming a SDR-typed 2n pollen.关键词
胡杨/花粉母细胞/减数分裂/微管骨架/胞质分裂/未减数2n花粉Key words
Populus euphratica/pollen mother cell/meiosis/microtubule cytoskeleton/cytokinesis/unreduced 2n pollen分类
生物科学引用本文复制引用
赵晨光,李燕杰,张平冬,康向阳..胡杨未减数2n花粉形成的细胞学机制[J].南京林业大学学报（自然科学版）,2015,(6):40-44,5.基金项目
国家自然科学基金项目 （）
