南京师大学报（自然科学版）Issue(3)：56-63,8.
NRAGE基因对小鼠海马神经元细胞分化的影响
Influence of NRAGE Gene on Mouse Hippocampus Nerve Cell Differentiation
摘要
Abstract
NRAGE,which belongs to MAGE family,plays an important role in the cell cycle,proliferation and apopto⁃sis. The influence of NRAGE on differentiation of neurons had been reported in two papers which indicated two opposite conclusions. These two articles based on PC12 cells,which derived from a pheochromocytoma of the rat adrenal medul⁃la. Our experiment isolated hippocampals of NRAGE knockout mice and took neonatal rat glial cells as feeder layer to obtain fetal mice primary neurons. Detected the influence of NRAGE gene on neurons differentiation by counting neu⁃rite/diameter of soma and the numbers of dendrites. The results showed that primary neuronal differentiations of wild-type and knockout fetal mice had no significant difference,indicating NRAGE gene has no effect on the neurons of mice.关键词
NRAGE/原代神经元细胞/分化/低密度培养Key words
NRAGE/primary neuron/differentiation/low-density cultures分类
生物科学引用本文复制引用
周笑天,王敏,刘振洲,李卉,仇海文,刘梅..NRAGE基因对小鼠海马神经元细胞分化的影响[J].南京师大学报（自然科学版）,2015,(3):56-63,8.基金项目
国家自然科学基金面上项目（31171135、30700200）、江苏高校优势学科建设工程资助项目 （）
