畜牧与饲料科学Issue(10)：1-3,7,4.
2014-2015年黑龙江省牡丹江地区犬细小病毒2型分子流行病学调查
Molecular Epidemiology of Canine Parvovirus-2 in Mudanjiang Area of Heilongjiang Province from 2014 to 2015
摘要
Abstract
In order to investigate the epidemiology of canine parvovirus-2 (CPV-2) in Mudanjiang area of Heilongjiang Province, a total of 40 stool samples were collected from dogs with diarrhea in Mudanjiang area of Heilongjiang Province from May 2014 to April 2015. The presence of CPV-2 in the samples was determined by PCR amplification of the partial VP2 gene (568 bp). The results revealed that 20 samples (50%) were positive for CPV-2, of which types of new 2a (Ser297Ala) and 2c accounted for 75% (15/20) and 25% (5/20), respectively. Sequence analysis revealed that 20 CPV-2 strains identified in our study showed 98.6%-100% nucleotide identity and 98.2%-100% amino acid identity. Phylogenetic analysis revealed that the identified 20 CPV-2 strains formed a specific group (A) with Chinese reference CPV-2 strains, of which the new 2a strains were divided into different clusters, when compared with CPV-2 reference strains from other countries. These data suggested that CPV-2 showed high prevalence in dogs with diarrhea in Mudanjiang area of Heilongjiang Province.关键词
犬/腹泻/CPV-2/CPV-2a/VP2基因/分子流行病学Key words
canine/diarrhea/CPV-2/CPV-2a/VP2 gene/molecular epidemiology分类
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耿雨菲,赵喜文,孙筱璇,边秀东,张立春,孙东波,王恩雨,滕井胜,李春秋,姚爽,王志慧,王欣宇,苏明俊,魏姗..2014-2015年黑龙江省牡丹江地区犬细小病毒2型分子流行病学调查[J].畜牧与饲料科学,2015,(10):1-3,7,4.基金项目
黑龙江省高校新世纪优秀人才计划（1252-NCET-016）。 （）
