农业科学研究2015，Vol.36Issue(3)：19-21,28,4.
FSH和LH对滩羊卵母细胞体外成熟培养的影响
Impacts of SH and LH on in vitro maturation of oocytes of Tan sheep
摘要
Abstract
To investigate the appropriate concentration of FSH and LH in TAN sheep oocyte maturation in vitro,TAN sheep oocytes were divided into four groups in vitro maturation culture: the control group,the FSH group,the LH group and the FSH&LH group. In the FSH group,the concentration of FSH was 10μg/ml,50μg/ml,100μg/ml,200μg/ml and 300 μg/ml respectively,in the LH group,the concentration of LH was 5μg/ml,10μg/ml,20μg/ml,50μg/ml and 100 μg/ml respectively,and the concentration of FSH and LH were respectively added 100 μg/ml and 20μg/ml in the FSH&LH group. The rates of polar body I(Pb I)expulsion was analyzed in the test. The results showed that the rate of oocytes maturation of the FSH&LH group was 64.64%,which was the highest rate in each group(P<0.05),the rate of oocytes maturation of the FSH&LH group was 64.64%,which the highest rate in each group(P<0.05),the FSH group including 100 μg/ml is better than the other four concentration gradient and the control group(P<0.05),the LH group including 20 μg/ml was better than the other four concentration gradient and the control group(P<0.05). Therefore,adding 100 μg/ml FSH and 20 μg/ml LH was the appropriate concentration of FSH and LH in TAN sheep oocyte maturation in vitro under this experimental condition.关键词
FSH/LH/滩羊/卵母细胞/体外成熟Key words
FSH/LH/oocyte/in vitro maturation/Tan sheep分类
农业科技引用本文复制引用
杨恕玲,马晓霞,张闫斌,黎永顺..FSH和LH对滩羊卵母细胞体外成熟培养的影响[J].农业科学研究,2015,36(3):19-21,28,4.基金项目
宁夏自然科学研究基金资助(NZ12150) （NZ12150）
项目名称:激素对滩羊卵母细胞体外培养的研究. （）
