农业环境科学学报Issue(9)：1659-1664,6.DOI:10.11654/jaes.2015.09.005
DDRT-PCR技术分析籽粒苋镉胁迫下相关基因的差异表达
Analysis of Differentially Expressed Genes of Amaranthus hypochondriacus Under Cadmium Stress by DDRT-PCR
晋海军 1胡洪利 1陈惠 1张世熔 2吴琦 1孙蓉 1唐自钟1
作者信息
- 1. 四川农业大学生命科学学院，四川 雅安 625014
- 2. 四川农业大学环境学院，成都 611130
- 折叠
摘要
Abstract
Amaranthus hypochondriacus has great potential for phytoremediation of cadmium(Cd)polluted soils. In this study, differential display reverse transcriptase polymerase chain reaction(DDRT- PCR) was used to screen differentially expressed genes in the roots , leaves and fruits of A . hypochondriacus under Cd stress. The novel bands were recovered and four differentially expressed cDNA fragments [EST (Expressed sequence tags)-1, EST-2, EST-3 and EST-4] were obtained. All ESTs had homology with known cDNA clones in the Genbank databases. The EST-1 was similar to partial sequences of Talinum paniculatum 26S ribosomal RNA gene(GB|HQ843466.1)with an identity of 99%, while the EST-2 showed similarity to partial sequences of Saxifraga mertensiana large subunit 26S ribosomal RNA gene (GB|AF036498.1)with an identity of 93%. The EST-3 was similar to complete cds of Escherichia coli strain TW 14359 DNA poly-merase Ⅲ alpha subunit(ECs0186)gene(GB|EU906049.1)and their identity was 97%. The EST- 4 was similar to Aspergillus oryzae RIB40beta-glucosidaseA(XM 001816779.2)andtheidentitywas99%.AfterBlastXcomparison,EST-1wassimilartohypothetical proteinMTR 5g051030[Medicagotruncatula](XP 003614386.1)withanidentityof26%,EST-2similartoputativesenescence-associat-ed protein[Cupressus sempervirens](GB|ACA30301.1)with an identity of 98%, EST-3 similar to DNA polymerase Ⅲ subunit alpha[Es-cherichiacoliOP50](ZP 06935700.1)withanidentityof100%,andEST-4similartobeta-glucosidase[AspergillusflavusNRRL3357] (XP 002383240.1)withanidentityof99%.Thesefourdifferentiallyexpressedgeneswereresponsivetocadmiumstressthroughregulating protein metabolism, DNA replication and glucose metabolism.关键词
籽粒苋/镉胁迫/DDRT-PCR技术Key words
Amaranthus hypochondriacus/cadmium stress/DDRT-PCR分类
资源环境引用本文复制引用
晋海军,胡洪利,陈惠,张世熔,吴琦,孙蓉,唐自钟..DDRT-PCR技术分析籽粒苋镉胁迫下相关基因的差异表达[J].农业环境科学学报,2015,(9):1659-1664,6.
