热带亚热带植物学报Issue(6)：601-613,13.DOI:10.11926/j.issn.1005-3395.2015.06.001
药用植物华重楼（黑药花科）叶绿体全基因组研究
Complete Chloroplast Genome of the Medicinal Plant Paris polyphylla var. chinensis (Melanthiaceae)
摘要
Abstract
In order to understand the characters of chloroplast genome (cp genome) in Paris polyphylla var. chinensis, the chloroplast genome (cp genome) of P. polyphylla var. chinensis was compared with those of 10 species within Liliales by using phylogenomics methods based on complete chloroplast genomes. The results showed that the cp genome of P. polyphylla var. chinensis was 158307 bp in length and display a typical quadripartite structure including two inverted repeat regions (IRA and IRB, 27473 bp), one small single-copy region (SSC, 18175 bp) and one large single-copy region (LSC, 85187 bp). It contained 115 unique genes, including 81 protein-coding genes, 30 tRNAs and 4 rRNAs. The genome structure, gene contents and arrangement of 10 Liliales species cp genomes were very similar. The cemA gene of P. polyphylla var. chinensis was pseudogene with poly(A) and CA SSR patterns after the start codon, and the loci of premature stop codons are different from those of Paris veticillata. In conclusion, the cp genome of P. polyphylla var. chinensis was conservative. The cemA structure and pseudogenization might play an important role in the evolution and phylogeny, and the location of the stop codons in cemA was useful for distinguishing P. polyphylla var. chinensis from P. veticillata.关键词
叶绿体全基因组/华重楼/黑药花科/百合目/cemA假基因化Key words
Complete chloroplast genome/Paris polyphylla var. chinensis/Melanthiaceae/Liliales/cemA pseudogenization引用本文复制引用
李晓娟,杨振艳,黄玉玲,纪运恒..药用植物华重楼（黑药花科）叶绿体全基因组研究[J].热带亚热带植物学报,2015,(6):601-613,13.基金项目
This work was supported by the National Natural Science Foundation of China (Grant No.30670132) （Grant No.30670132）
