摘要
Abstract
To study chronic thromboembolic pulmonary hypertension ( chronic thrombo embolic pulmonary hypertension, CTEPH) Changes in the expression levels of inflammatory cytokines in order to assess the relationship between pulmonary vascular remode-ling inflammatory response. Methods:64 male Wistar rats were randomly divided into 8 groups of 8: initial control group ( IC group) , embolism 3 d group, one week group, 2 weeks, 4 weeks, 8 weeks group, 12 weeks group (D3, W1, W2, W4, W8, W12), the terminal group ( EC group). Preparation of blood of about 0. 5 mm size autologous thrombus, jugular vein injection of 27 to 30 autologous thrombus, the second injection the same number thrombosis two weeks after two intramuscular gentamicin prevention of infection after sur-gery, throughout the intraperitoneal injection of tranexamic acid. Up to the target date after each set of experiments, the rats measured mean pulmonary arterial pressure ( mPAP) , pulmonary relative thickness ( PAMT) , wall area / total area ( WA / TA) percentage immu-nohistochemistry vascular endothelial growth factor ( VGEF) , tumor necrosis factor ( TNF-α) expression. Results:After the first embo-lization mPAP increased slightly, no significant difference (P> 0. 05) with the control group, after the second embolization mPAP signifi-cantly increased with time (all P <0. 01);PAMT , WA / TA in four weeks after the extension was significantly increased over time (4 weeks group P <0. 05,8 week, 12 weeks group P <0. 01);serum at different times VGEF, TNF-αthan the initial value of the control group and terminal control group were significantly higher, P <0. 01, the difference was statistically significant, which reached its peak W1, then declined again after the Second embolization W4 peaked, then maintain a high level. Correlation analysis showed that, VGEF positively correlated with TNF-α and remodeling index showed (P <0. 01), VGEF TNF-αexpression was positively correlated (P <0. O1). Conclusion:VGEF and TNF-α may be involved in both chronic thromboembolic pulmonary hypertension, the pathophysiologi-cal process of pulmonary vascular remodeling, inflammation is an important mechanism of chronic pulmonary hypertension.关键词
血栓栓塞/肺动脉高压/炎症反应/肺血管重构Key words
Thromboembolism/Pulmonary hypertension/Inflammation/Pulmonary vascular remodeling分类
医药卫生
