山东农业大学学报(自然科学版)Issue(5):652-657,6.
盐胁迫下小麦幼苗相关性状QTL加性及其上位性效应分析
Analysis on Quantitative Trait Loci Additive and Epistatic Effects of Wheat Seedling Under Salt Stress
摘要
Abstract
Seedling height and dry seedling weight are important indexes to evaluate salt tolerance of wheat seedlings. To detect quantitative trait loci (QTL) associated with seedling height and dry seedling weight at seedling stage in wheat, a set of 168 doubled haploid (DH) lines derived from the cross between Huapei 3 and Yumai 57 was treated with distilled water (CK), 50 and 100 mmol/L of NaCl. Based on inclusive composite interval mapping (ICIM) method, we identified 16 additive QTLs and 17 pairs of epistatic QTLs for seedling height and dry seedling weight under CK and salt stress. A total of eight QTLs for seedling height were detected distributed on chromosomes 2A, 2D, 3B, 4D, 6B and 7B, and explained phenotypic variation ranging from 3.38%to 22.96%. A total of eight QTLs for dry seedling weight were detected distributed on chromosomes 1A, 1B, 2B, 2D, 4D and 5B, which accounted for 4.53-9.10%of the phenotypic variation .The QDSW1A for dry seedling weight expressed both in CK and salt stress, which could be used in marker-assisted selection in wheat breeding programs. The results indicate that both additive effects and epistatic effects are important genetic bases for wheat seedling traits.关键词
小麦(Triticum aestivum L.)/幼苗/盐胁迫/加性效应/上位性效应Key words
Wheat (Triticum aestivum L.)/seedling stage/salt stress/additive effects/epistatic effects分类
农业科技引用本文复制引用
吴儒刚,陈广凤,李冬梅,田纪春..盐胁迫下小麦幼苗相关性状QTL加性及其上位性效应分析[J].山东农业大学学报(自然科学版),2015,(5):652-657,6.基金项目
国家自然科学基金(31171554) (31171554)
国家转基因生物新品种培育重大专项(2013ZX08002003) (2013ZX08002003)
山东省农业良种工程项目(鲁农良种字【2014】96号) (鲁农良种字【2014】96号)