山东农业大学学报（自然科学版）Issue(5)：676-681,6.
牛皮杜鹃嫩茎段试管内高效再生方法
An Efficient Plant Regeneration Method of Rhododendron chrysanthum Pall. Tender Stem Segments in Vitro
摘要
Abstract
In this study, the young stems of Rhododendron chrysanthum were used as explants, the main factors with different levels of axillary sprouting, shoots growth and shoots rooting were investigated through uniform design experiments. To explore a method of efficient plantlet regeneration in vitro. The results showed that the basic medium+KT 0.13~0.14 mg·L-1+IBA 0.05~0.08 mg·L-1+NAA 0.02~0.05 mg·L-1+GA3 2.70~2.90 mg·L-1 was the most suitable for axillary sprouting, shoots growth and shoots rooting, the rate of plantlet regeneration was 99.2%. Stems each with one node were cut from regenerated shoots and cultured for propagation, and a 6-fold proliferation rate was achieved within 28 days. The survival rate of regenerated plants was more than 96.0%. Efficient plantlet regeneration system of Rhododendron chrysanthum have been successfully established, which is suitable to industrialized seedling for Rhododendron chrysanthum.关键词
牛皮杜鹃/嫩茎/均匀设计法/植株再生Key words
Rhododendron chrysanthum Pall./tender stem segments/uniform design method/plantlet regeneration分类
生物科学引用本文复制引用
顾地周,杨丽娟,王曼宁,刘宇,赵明明,沈红梅,朱俊义,周繇..牛皮杜鹃嫩茎段试管内高效再生方法[J].山东农业大学学报（自然科学版）,2015,(5):676-681,6.基金项目
吉林省科技发展计划资助项目(20130206060NY （）
20121705 （）
20150307010NY) （）
