药学研究Issue(10)：574-576,610,4.
二甲双胍格列吡嗪片中盐酸二甲双胍含量测定方法改进
Improvement of assay method of metformin hydrochloride in Metformin and Glipizide Tablets
王长丹 1臧恒昌 2左艳红1
作者信息
- 1. 山东大学药学院，山东 济南 250012
- 2. 合肥立方制药股份有限公司，安徽 合肥 230088
- 折叠
摘要
Abstract
Objective To establish an HPLC method for the determination of metformin hydrochloride in Metformin and Glipizide Tablets. Methods The separation was performed on C18 column(4. 6 mm × 150 mm,5 μm),and the mobile phase was composed of 0. 05% sodium heptane - 1 - sulphonate solution(with 10% phosphoric acid solution to adjust pH value to 4. 0)- acetonitrile(84:16)with the flow rate of 1. 0 mL·min - 1 . The detection wavelength was 233 nm. The col-umn temperature was 30 ℃ . Results The linear range of metformin hydrochloride was 10. 02 ~ 30. 06 μg·mL - 1(r =0. 999 9). The average recovery rate was 101. 0% with RSD of 0. 66%(n = 9). Conclusion The established method was better and more accurate than the original national registered standard specificity.关键词
高效液相色谱法/二甲双胍格列吡嗪片/盐酸二甲双胍Key words
HPLC/Metformin and Glipizide Tablets/Metformin hydrochloride分类
医药卫生引用本文复制引用
王长丹,臧恒昌,左艳红..二甲双胍格列吡嗪片中盐酸二甲双胍含量测定方法改进[J].药学研究,2015,(10):574-576,610,4.
