山东农业科学Issue(10)：8-12,5.DOI:10.14083/j.issn.1001-4942.2015.10.002
黄曲霉漆酶基因 HIGS 载体的构建及对花生的转化
Construction of HIGS Vectors for Laccase Genes from Aspergillus flavus and Its Transformation to Peanut
摘要
Abstract
Aflatoxin contamination is a severe problem adversely affecting peanut food safety.Breeding a new peanut variety resistant to Aspergillus flavus is a lengthy process through conventional means,and the re-sults are far from satisfaction.Host -induced gene silencing (HIGS),as a novel technology for RNA interfer-ence,provides possibilities for creating disease -resistant crop plants.However,there is no reports on pea-nut.To breed new A.flavus -resistant peanut through genetic engineering,the HIGS vectors containing 2 lac-case genes,Lac1 and Lac2,were constructed and then were transformed into an A.flavus -susceptible peanut cultivar Yueyou 20.PCR -positive transgenic peanut seeds with Lac1 gene were obtained.According to the HIGS principle,if the transgenic peanut is infected by A.flavus,the laccase gene expression will be inhibited by dsRNA,thus the resistance of peanut to A.flavus will be induced.Based on this principle,whether the transgenic seeds had resistance to A.flavus infection would be identified next.The study layed foundations for exploring the relationship between laccase gene and A.flavus pathogenicity in peanut,and provided a new way for breeding peanut cultivars with A.flavus resistance.关键词
花生/黄曲霉/漆酶基因/寄主诱导的转基因沉默(HIGS)/遗传转化Key words
Peanut/Aspergillus flavus/Laccase gene/Host -induced gene silencing (HIGS)/Genetic transformation分类
农业科技引用本文复制引用
孙全喜,王云云,王秀贞,唐月异,吴琪,张青云,曹广英,王传堂..黄曲霉漆酶基因 HIGS 载体的构建及对花生的转化[J].山东农业科学,2015,(10):8-12,5.基金项目
国家花生产业技术体系（CARS －15）；山东省农业科学院重大科技成果培育计划项目 （）
