山东医药Issue(38)：14-17,4.DOI:10.3969/j.issn.1002-266X.2015.38.005
苯丁酸钠联合吉非替尼对肺腺癌 A549细胞增殖、迁移的影响及机制
Effects of sodium phenylbutyrate combined with gefitinib on proliferation and migration of lung adenocarcinoma A549 cells
摘要
Abstract
Objective To study the effects of sodium phenylbutyrate combined with gefitinib on proliferation and mi -gration of lung adenocarcinoma A 549 cells and to investigate the mechanism .Methods The lung adenocarcinoma A 549 cells were cultured and divided into the blank control group ( treated with DMEM medium without FBS ) , gefitinib group (treated with 1 mmol/L gefitinib), sodium phenylbutyrate group (treated with 5 μmol/L sodium phenylbutyrate ) and the combined treatment group ( treated with 1 mmol/L gefitinib and 5 mol/L sodium phenylbutyrate ) .After culture for 24 h, the OD450 was detected by MTT method and the cell cycle distribution was detected by flow cytometry .The expression of ap-optosis-related genes survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP-9 was detected by RT-PCR.The cell migration was assayed by scratch test .Results Compared with the blank control group , the OD450 , cell proportion in S phase, cell mobility and the expression of survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP-9 mRNA were decreased, and the cell proportion in G0/G1 phase was increased in the combined treatment group , sodium phenylbutyrate group and gefitinib group (all P<0.05).Compared with the sodium phenylbutyrate group and gefitinib group , the OD450, cell proportion in S phase , cell mobility and the expression of survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP-9 mRNA were decreased , and the cell proportion in G 0/G1 phase was increased in the combined treatment group ( all P<0.05).Conclusion The sodium phenylbutyrate and gefitinib can both inhibit the proliferation and migration process of lung adenocarcinoma , which may be related with the decreased expression of survivin , BRAF, Notch1, MMP-1, MMP-2, MMP-3 and MMP9 mRNA.The combined application of the two drugs has synergistic effect .关键词
肺腺癌/苯丁酸钠/吉非替尼/细胞增殖/细胞周期/细胞迁移/细胞凋亡/凋亡相关基因Key words
pulmonary adenocarcinoma/sodium phenylbutyrate/gefitinib/cell proliferation/cell migration/apopto-sis/apoptosis-related genes分类
医药卫生引用本文复制引用
张振安,张静,张风林,袁亚军..苯丁酸钠联合吉非替尼对肺腺癌 A549细胞增殖、迁移的影响及机制[J].山东医药,2015,(38):14-17,4.基金项目
河北省自然科学基金资助项目（20101112）。 （）
