山东医药Issue(39)：16-18,3.DOI:10.3969/j.issn.1002-266X.2015.39.006
七氟醚预处理对大鼠肝缺血再灌注致肺损伤的干预作用及机制探讨
Intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and its mechanism
摘要
Abstract
Objective To observe the intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and to explore its mechanism .Methods A total of 30 healthy Wistar rats were randomly divided into sham-operation group , the model group and the sevoflurane group , with 10 rats in each group .Models of liver ischemia reperfusion were established in the model group and sevoflurane group , and rats of the sevoflurane group were given inhala-tion of sevoflurane for 30 min before modeling; while in the sham-operation group , only porta hepatis was separated but blood supply was not blocked .Rats were scarified at 60 min after reperfusion , and the serum alanine aminotransferase ( ALT) , aspertate aminotransferase ( AST) and tumor necrosis factor-α( TNF-α) were detected; part of right upper lobe was used to measure the wet/dry weight ratio ( W/D ratio) and part was used to make paraffin section for pathological ob-servation;and left lung tissue was taken to determine malondialdehyde ( MDA) and MMP-9 mRNA.Results Lung tissue of the sham-operation group was normal in structure under microscopy , while damage degree of lung tissue in the sevoflu-rane group was reduced as compared with that of the model group .Serum ALT, AST and TNF-αof the model group and sevoflurane group were increased as compared with those of the shan-operation group (all P<0.05); while serum ALT, AST and TNF-αof the sevoflurane group was lower than that of the model group (all P<0.05).W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the model group and sevoflurane group were increased as compared with those of the sham-operation group ( all P<0.05 ); W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the sevoflurane group were lower than those of the model group (all P<0.05).Con-clusion Sevoflurane can alleviate the pulmonary injury caused by liver ischemia reperfusion , whose mechanism may be re-lated to the lower levels of TNF-αand inhibition of MMP-9 mRNA expression, etc.关键词
七氟醚/肝缺血再灌注/肺损伤/动物实验模型Key words
sevoflurane/liver ischemia reperfusion/lung injury/animal experiment model分类
医药卫生引用本文复制引用
王晓丽,徐桂萍,管小萌..七氟醚预处理对大鼠肝缺血再灌注致肺损伤的干预作用及机制探讨[J].山东医药,2015,(39):16-18,3.基金项目
国家自然科学基金资助项目（81160016）。 （）
