山东医药Issue(42)：16-19,4.DOI:10.3969/j.issn.1002-266X.2015.42.006
新型香豆素类酰腙-铜配合物对肺腺癌A549细胞的体内外抑制作用
Inhibitory effect of new copper (Ⅱ) complex with coumarin derivatives on lung cancer cells A549 in vivo and vitro
摘要
Abstract
Objective To observe the inhibitory effect of a new copper (Ⅱ) complex with coumarin derivatives ( CCCD) on lung cancer cell line A549 in vivo and in vitro and to investigate the mechanism .Methods The lung cancer A549 cells were cultured and were treated with 5, 10, 20, 30, 50, 80, 120, 160μmol/L CCCD for 72 h, then the inhibi-tion rate ( IR) of tumor cells was calculated by using MTT assay .A549 cells were divided into the intervention group which was treated with 10 , 20 and 40μmol/L CCCD and the control group which was treated with PBS .The apoptosis and the ap-optosis rate were analyzed by flow cytometry , and the expression of caspase-3 was detected by Western blotting .Further-more, 18 tumor-bearing nude mice were divided into the observation group 1, observation group 2 and the control group , which were treated with 4, 8 mg/kg CCCD and PBS once a week for three weeks , and then they were executed to detect the tumor volume and tumor inhibition rate .After that, the tumor tissues were obtained to detect the apoptosis and apoptosis in-dex (AD) by TUNEL method.Results The inhibition rates of A549 cells were 8.80%, 16.52%, 37.24%, 55.75%, 77.22%, 87.16%, 95.25%and 98.70%after being treated with 5, 10, 20, 30, 50, 80, 120 and 160μmol/L CCCD. The IR showed an increased tendency with the increased concentration of the drugs .And being after treated with 10, 20 and 40 μmol/L CCCD, the apoptosis rate and expression level of caspase-3 in the intervention group were significantly higher than those of the control group (all P<0.05).The AD in the observation groups 1, 2 and the control group were 16.83%±8.44%, 24.65%±11.24%and 3.30%±2.12%(all P<0.05).And the tumor inhibition rates in the observation groups 1 and 2 were 51.80%and 56.78%, respectively.Conclusions CCCD may inhibit the growth of A549 cells in vi-vo and in vitro and enhance the apoptosis , and its mechanism might be related to inducing tumor cell apoptosis through acti-vating caspase-3.关键词
肺癌/香豆素类酰腙酰腙-铜配合物/细胞增殖/细胞凋亡/半胱氨酸天冬氨酸蛋白酶3Key words
lung carcinoma/copper (Ⅱ) complex with coumarin derivatives/cell proliferation/apoptosis/caspase-3分类
医药卫生引用本文复制引用
陆勤,欧秋霞,朱文娇,朱涛峰..新型香豆素类酰腙-铜配合物对肺腺癌A549细胞的体内外抑制作用[J].山东医药,2015,(42):16-19,4.基金项目
江苏大学医学临床科技发展基金项目（JLY20140065）。 （）
