山东医药Issue(43)：20-23,4.DOI:10.3969/j.issn.1002-266X.2015.43.007
表观遗传修饰肿瘤细胞疫苗皮下注射对仓鼠胰腺癌的抗肿瘤效应
Anti-tumor effects of tumor cell vaccine by epigenetic modification on pancreatic cancer of hamster
摘要
Abstract
Objective To observe the anti-tumor effects of tumor cell vaccine by epigenetic modification on pancreatic cancer of hamster through subcutaneous injection. Methods The hamster pancreatic cancer models were set up by subcu-taneous injection of N-nitrosobis-2-oxopropylamin. We removed the pancreatic tumor, chopped, and took 0. 5 cm × 0. 5 cm × 0. 5 cm tumor tissue to transplant into groin skin of healthy hamsters, and then the hamster subcutaneous transplanted tumor models were set up. In addition, PANC-1 cells were treated by histone deacetylase inhibitors and methyl transferase inhibitor epigenetic modification to make the tumor cell vaccine. Meanwhile, the control cell vaccine was set up. Twelve hamster pancreatic cancer xenografts were divided into two groups, 6 in each group: the vaccinated group and the control group which were respectively treated with tumor cell vaccine and control cell vaccine by subcutaneous injection. The tumor growth and the expression of cyclooxyg enase-2 (COX-2), 5-LOX, GAL-1, GAL-3, MHC Ⅰ and MHC Ⅱ of the two groups were observed. Results In the vaccinated group, the tumor size ( mean size 0. 39 cm) was decreased with the prolonged time, but in the control group, the tumor size ( mean size 0. 82 cm) was increased with the prolonged time. Tumor size was statistical different between the two groups (P<0. 05). The expression of COX-2, 5-LOX, GAL-1 and GAL-3 was lower in the vaccinated group as compared with that of the control group, and MHCⅠ, MHCⅡexpression was higher than that of the control group, the difference was statistically significant (all P<0. 01). Conclusion The epige-netic modification tumor vaccine can effectively delay the growth of transplanted tumor, reduce the expression of COX-2, 5-LOX, GAL-1 and GAL-3, and up-regulate the expression of MHC Ⅰ and MHC Ⅱ.关键词
胰腺肿瘤/胰腺癌/胰腺癌PANC-1细胞株/肿瘤细胞疫苗/表观遗传修饰/免疫逃逸/动物实验Key words
pancreatic neoplasms/pancreatic carcinoma/pancreatic carcinoma cell line PANC-1/tumor vaccine/epigenetic modification/immune escape/animal experiment分类
医药卫生引用本文复制引用
张海峰,曹维,周国雄,陈海琴,陈卫昌..表观遗传修饰肿瘤细胞疫苗皮下注射对仓鼠胰腺癌的抗肿瘤效应[J].山东医药,2015,(43):20-23,4.基金项目
国家自然科学基金资助项目(81072028) （81072028）
江苏省创新团队医学领军人才项目(LJ201135)。 （LJ201135）
