山东医药Issue(44)：4-7,4.DOI:10.3969/j.issn.1002-266X.2015.44.002
肝外胆管癌患者胆汁上清液及沉淀物中K-ras、p53基因突变检测及意义
Detection and significance of K-ras and p53 gene mutations in supernatant and sediment of bile in patients with extrahepatic cholangiocarcinoma
摘要
Abstract
Objective To detect the K-ras and p53 gene mutations both in the supernatant and sediment of bile sam-ples from patients with extrahepatic cholangiocarcinoma ( EHC) and to investigate its significance .Methods Fifty-seven patients with EHC and 42 patients with benign bile duct stricture were surveyed .The supernatant and sediment in their bile was collected by duodenal endoscopy .Polymerase chain reaction-single-strand conformation polymorphism ( PCR-SSCP ) and DNA direct sequencing were performed to detect K-ras mutations and p53 mutations both in the supernatant and sedi-ment of bile samples .Results No gene mutations were detected in 42 patients with benign bile duct stricture in either su-pernatant or sediment of bile .The mutation rate of K-ras gene of patients with EHC was 77.19%(44/57), 66.67%(38/57) in the bile supernatant and 59.65%(34/57) in the corresponding sediment, respectively (P>0.05).The mutation rate of p53 gene of patients with EHC was 70.18% (40/57), 63.16% (36/57) in the bile supernatant and 45.61%(25/57) in the corresponding sediment, respectively (P<0.05).The total mutation rate of combined K-ras and p53 genes in the supernatant and sediment of bile samples from patients with EHC was 85.97%(49/57).Conclusions K-ras and p53 mutations were detected both in the supernatant and sediment of bile from patients with EHC .The combined detec-tion of K-ras and p53 mutations improved the positive rate .The detection of K-ras and p53 mutations may be used in the differential diagnosis of extrahepatic cholangiocarcinoma and benign bile duct stricture .关键词
肝外胆管癌/胆管良性狭窄/胆汁/p53基因/K-ras基因Key words
extrahepatic cholangiocarcinoma/benign bile duct stricture/bile/p53 gene/K-ras gene分类
医药卫生引用本文复制引用
张双霞,张国梁,鲍文漪..肝外胆管癌患者胆汁上清液及沉淀物中K-ras、p53基因突变检测及意义[J].山东医药,2015,(44):4-7,4.基金项目
天津市卫生局基金资助项目（06KR07）。 （）
