山西中医学院学报Issue(4)：19-21,24,4.
右归饮促异体骨髓细胞向成骨分化防治辐照后骨量丢失机制的实验研究
Experiment mechanism study on bone loss prevention caused by irradiation with Youguiyin promoted the osteogenic differentiation of the allogeneic bone marrow stem cell
摘要
Abstract
Objective:To study the influence of Youguiyin on the osteogenic differentiation of the allogeneic bone marrow stem cell in mice so as to reveal the mechanism of Youguiyin preventing bone loss caused by irradiation. Methods:Eighty BALB/c male mice with age of 8-week were prepared. 32 mice of them were used to extract bone marrow stem cell for transplantation. 48 mice were randomly divided into control group,irradiation group,bone marrow cell(BM)transplanting group,Youguiyin high dose group,middle dose group,low dose group. The mice in irradiation group were exposed to 6.0 Gy gamma irradiation. The mice in BM transplanting group were exposed 6.0 Gy irradiation and received bone marrow cells. The mice in high dose group,middle dose group and low dose group were exposed to 6.0 Gy irradiation and received bone marrow cells and gavaged with Youguiyin of 0.03 mL/g,0.02 mL/g,0.01 mL/g,respectively. After 2 w,serum alkaline phosphatase (ALP),bone volume of femur bone (bone mineral density)and pathological tissue changing were observed. Results:Compared with the irradiation group and BM transplanting group,bone mineral density in high dose group was significantly increased (P<0.01). Middle dose group had a statistically difference with the irradiation group and BM transplanting (P < 0.05).Compared with the irradiation group,in high does group,middle dose group and control group ALP had increased (P<0.01)and high does group had a statistically difference with low dose group and BM transplanting group (P<0.05). Femur pathology:After irradiating,the structure of myeloid tissue become mass and monocyte was rare in the bone marrow and a part of tissue were degenerated. Bone trabeculas reappeared and bone marrow mononuclear cells were recovered,when injected with bone marrow stem cell. In addition,high dose group has strong and well ordered trabecular,less fat cavitations. Conclusion:Youguiyin can promote the osteogenic differentiation of the allogeneic bone marrow stem cell to cure the bone loss caused by irradiation.关键词
右归饮/骨髓细胞/成骨分化/电离辐射Key words
Youguiyin/bone marrow cell/osteogenic differentiation/ronizing radiation分类
医药卫生引用本文复制引用
夏臣杰,尹利明,黄杰烽,陈俊杰,赵凯,杜文喜..右归饮促异体骨髓细胞向成骨分化防治辐照后骨量丢失机制的实验研究[J].山西中医学院学报,2015,(4):19-21,24,4.基金项目
国家自然科学基金资助项目（81202709；30873276）；浙江省科学基金资助项目（Y2111092）；浙江省重点科技创新团队计划基金资助项目（2011R50022-07）；浙江省中医药管理局基金资助项目 （）
