山西中医学院学报Issue(4)：25-28,44,5.
糖尿病大鼠模型的建立及相关蛋白组学的实验研究
Establishment of the diabetic rat models and analysis of serum proteomics
陈红谨 1刘小溪 2连捷 3杨宇峰 4石岩4
作者信息
- 1. 山西中医学院，山西太原030024
- 2. 辽宁中医药大学附属医院，辽宁沈阳110032
- 3. 大连市骨科医院，辽宁大连116011
- 4. 辽宁中医药大学，辽宁沈阳110847
- 折叠
摘要
Abstract
Objective:To study the establishment of the diabetic rat models and the change of serum proteomics so as to exploring the pathophysiological mechanism of diabetes from the level of protein molecules. Methods:The diabetic rat models were established by streptozotocin(STZ)and high fat-glucose diet. The serum proteomics was analyzed with isotope internal standard dilution technique,high performance liquid chromatography/tandem mass spectrometry (LC-MS/MS). Results:From the second week,blood glucose in model group was significantly higher than that in normal group(P<0.05). For tenth week,the blood glucose of model group had basically reached the standard of diabetes model (FPG>16.7 mmol/L). There was significant difference in expression of 50 kinds of proteins between normal group and model group (17 kinds of proteins were up-regulated more than 1.5 times,including MHCⅡ transactivator,major urinary protein,LIM domain protein,etc;50 kinds of proteins down-regulated less than 0.75 times,including carnitine acetyltransferase,polymeric immunoglobulin receptor precursor,etc). Conclusion:The diabetic rat models were established successfully. Pathological mechanism of diabetes may be related to disorders of immune and glucolipid metabolism.关键词
糖尿病/链脲佐菌素/蛋白组学/动物模型Key words
diabetes mellitus/streptozotocin/proteomics/animal models分类
医药卫生引用本文复制引用
陈红谨,刘小溪,连捷,杨宇峰,石岩..糖尿病大鼠模型的建立及相关蛋白组学的实验研究[J].山西中医学院学报,2015,(4):25-28,44,5.
