山西中医学院学报Issue(5)：16-17,2.
脊髓神经细胞缺血再灌注损伤模型的建立及鉴定
Establishment and qualification of the spinal cord nerve cells ischemia-reperfusion animal model
摘要
Abstract
Objective:To establish animal model of spinal cord nerve cells ischemia-reperfusion in rats. Methods:The rats on 15th day of gestation were killed,spinal cord nerve cells of fetal rat was taken out. Then nerve cells were separated and cultured. The spinal nerve cells ischemia-reperfusion model was established by damaging induced by glutamate. The ap-propriate concentration and duration of glutamate were determined by cell viability,content of LDH and MDA in culture medium. Results:It was easy to establish the spinal nerve cell ischemia-reperfusion model by exposed to 200 μmol/mL glutamate for 15 min. Conclusion:This method is convenient and repeatable.关键词
缺血再灌注/脊髓神经细胞/动物模型Key words
ischemia-reperfusion/spinal cord nerve cells/animal model分类
医药卫生引用本文复制引用
林翔,王荣茂,聂光瑞,张俐..脊髓神经细胞缺血再灌注损伤模型的建立及鉴定[J].山西中医学院学报,2015,(5):16-17,2.基金项目
福建省自然科学基金青年课题项目 （）
