上海农业学报Issue(5)：1-5,5.DOI:10.15955/j.issn1000-3924.2015.05.01
利用分子标记辅助选育香型优质粳稻新种质
Breeding of new fragrant japonica germplasm with high quality by molecular marker-assisted selection
摘要
Abstract
To breed fragrant rice with high quality,japonica variety ‘Wuxiang 075’was used as a fragrance gene donor and high quality rice variety ‘Jinfeng’as a recipient.Allele specific amplification and KOH were utilized to identify homozygous fragrant,homozygous non-fragrant,and heterozygous non-fragrant individuals.A fragrant rice germplasm named ‘Xiangjing 1305’with high quality was obtained through six generational agro-nomic traits selection.The quality of ‘Xiangjing 1305’was analyzed and tested by Rice Product Quality Supervi-sion and Inspection Center of Ministry of Agriculture in 2013.The brown rice rate,milled rice rate and head rice rate was 84.2%,75.8% and 73.8% respectively.Its chalky rice rate was 13% with 2.0 chalkiness degree,and transparency was 1 with 1.0 alkali digestion value.The gel consistency was found to be 70 mm with 15.3% amy-lose content and 9.3% protein content.The grain quality of the new germplasm reached the second class of na-tional edible rice standard.After cultivated in Jinshan District,Chongming County and Shangshi contemporary agricultural park in 2013—2014,the yields of ‘Xiangjing 1305’were at high and stable level of 9 487.50—9 685.50 kg?hm2 ,which increased with a range of 3.97%—7.23% than the control (‘Xiushui 128’and‘Xiushui 134’)and achieved significant or extremely significant level.By combination method of marker-assisted selection and conventional breeding,fragrant rice germplasms could be easily identified and obtained.关键词
水稻/香味基因/分子标记辅助选择/等位基因特异扩增Key words
Rice/Fragrant gene/Marker-assisted selection/Allele specific amplification引用本文复制引用
赵志鹏,李刚,施标,李茂柏,王新其,吴书俊,曹黎明,张建明..利用分子标记辅助选育香型优质粳稻新种质[J].上海农业学报,2015,(5):1-5,5.基金项目
上海市农业科学院院发基金项目［农科发2013（06）］；上海市科技兴农重点攻关项目［沪农科攻字（2015）第1-3-2号］；江苏省植物功能基因组学重点实验室开放课题 （）
