世界中医药Issue(10)：1578-1581,4.DOI:10.3969/j.issn.1673-7202.2015.10.033
接骨丹介导成骨细胞增殖促进骨折愈合的观察
The Observation of Bone-Joining Elixir Mediated Osteoblast Proliferation to Promote Fracture Healing
摘要
Abstract
Objective:To observe the effect of Bone-Joining Elixir on New Zealand newborn rabbit osteoblast proliferation,and ex-plore the promoting fracture healing mechanism of Bone-Joining Elixir.Methods:Conventional grade New Zealand big and white newborn rabbits were put to death by ear venous air embolism,the craniums were taken down in aseptic conditions,and the osteo-blasts were gained by collagenase type Ⅰ digestion method for in vitro culture,identification and passages.After identification, the second generation of osteoblasts was randomly divided into normal control group and experimental group.And the normal con-trol group was cultured in normal rabbit serum,the experimental group was cultured in Bone-Joining Elixir medicated serum,MTT method was taken to observe the cell growth curves of the two groups.After the intervention for 48 h,the two serums were observed to achieve the influence of Bone-Joining Elixir on the expression of osteoblast alkaline phosphatase and collagen typeⅠ under the inverted microscope,and to achieve the influence of Bone-Joining Elixir on the expression of osteocalcin in the supernate in the os-teoblast culture by Elisa method.Results:The osteoblast culture system in vitro has been successfully obtained and established by the collagenase type Ⅰ digestion method,MTT observation indicated the experimental group show significant promotion on the pro-liferation of osteoblasts,and the inverted microscope indicated the expression of alkaline phosphatase and collagen type Ⅰ in the experimental group was superior to the normal control group,and the osteoblast was promoted to secrete osteocalcin.Conclusion:The Bone-Joining Elixir can promote the healing of fracture,the mechanism may be related to the promotion of the expression of al-kaline phosphatase,collagen type Ⅰ and osteocalcin in osteoblasts to help osteoblasts proliferate and differentiate.关键词
接骨丹/含药血清/成骨细胞/骨折愈合Key words
Bone-Joining Elixir/Medicated Serum/Osteoblast/Fracture Healing分类
医药卫生引用本文复制引用
翟献斌,吴方云..接骨丹介导成骨细胞增殖促进骨折愈合的观察[J].世界中医药,2015,(10):1578-1581,4.基金项目
山东省中医药科技发展计划项目 （）
