色谱Issue(10)：1097-1103,7.DOI:10.3724/SP.J.1123.2015.03021
超高效液相色谱-四极杆飞行时间质谱法快速筛查水产品中15种碱性合成色素
Rapid screen of 15 basic artificial dyes in fishery products by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry
摘要
Abstract
A new method was established for the simultaneous determination of 15 basic artifi-cial dyes in fishery products by ultra performance liquid chromatography coupled with quadru-pole-time of flight mass spectrometry ( UPLC-Q-TOF MS ). The analytes in fishery products were extracted with acetonitrile containing 10%( v/v)acetic acid,then purified by dispersive solid phase extraction with C 18-silicone adsorbent. The compounds were separated by a C 18 col-umn with acetonitrile and 0. 1%( v/v)formic acid-5 mmol/L ammonium acetate solution as the mobile phases, and measured by UPLC-Q-TOF MS with electrospray ionization in positive mode. As a result,the limits of quantification( LOQs,S/N=10)of the 15 target compounds were 0. 1-100 μg/kg in fishery products. The 15 analytes behaved linearly in their respective ranges with the correlation coefficients no less than 0. 993. The average recoveries of the 15 analytes spiked at three levels were ranged from 80. 60% to 107. 37% with the RSDs of 3. 33%-6. 69%( n=6). This method is suitable for routine qualitative and quantitative analyses of the 15 basic artificial dyes in fishery products due to its fastness,simplicity and relatively high sensi-tivity.关键词
超高效液相色谱-四极杆飞行时间质谱/C18复合硅胶基质萃取管/碱性合成色素/水产品/快速筛查Key words
ultra performance liquid chromatography-quadrupole-time of flight mass spectrom-etry(UPLC-Q-TOF MS)/C18-silicone solid phase extraction column/basic artificial dyes/fish-ery products/rapid screening分类
化学化工引用本文复制引用
黄鹂,戴意飞,王萍亚,周勇,赵巧灵,蒋玲波,罗海军..超高效液相色谱-四极杆飞行时间质谱法快速筛查水产品中15种碱性合成色素[J].色谱,2015,(10):1097-1103,7.基金项目
国家科技部公益专项（201210060）；舟山市公益类科技项目（2013C31050）． （）
