色谱Issue(11)：1169-1174,6.DOI:10.3724/SP.J.1123.2015.08010
分散固相萃取净化-超高效液相色谱-串联质谱法测定鸡蛋和鸡肉中金刚烷胺和金刚乙胺残留
Determination of amantadine and rimantadine residues in egg and chicken samples by dispersive solid phase extraction purification-ultra high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method was developed for the determination of residual amantadine and rimanta-dine in eggs and chickens by dispersive solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry. Egg and chicken samples were extracted with ammonia water-acetonitrile(2:98,v/v). The extraction solution was dried to 1 mL under nitro-gen,and then purified by dispersive solid phase extraction method with C18 and NH 2 sorbents. After purification,the extraction solution was filtered through a filter. The target compounds were analyzed by ultra high performance liquid chromatography-tandem mass spectrometry ( UHPLC-MS/MS)on a ZORBAX C18 column using a mixture of 1 mmol/L ammonium acetate solution( containing 0. 1%( v/v ) formic acid ) and methanol as mobile phases with gradient elution. The mass spectrometer was operated under multiple reaction monitoring( MRM)mode in positive mode. The good linearities were obtained for amantadine and rimantadine at a con-centration range of 0. 15-10. 0 μg/L. The limits of detection for amantadine and rimantadine were all 0. 05 μg/kg,and the limits of quantification were 0. 20 μg/kg. The recoveries of aman-tadine and rimantadine in eggs and chickens at three spiked levels(0. 2,1. 0 and 2. 0 μg/kg) were in the range of 89%-108% with the relative standard deviations of 5. 0%-8. 6%. The results demonstrated that the method is suitable for the determination of amantadine and rimantadine in eggs and chickens.关键词
分散固相萃取/超高效液相色谱/串联质谱/金刚烷胺/金刚乙胺/鸡蛋/鸡肉Key words
dispersive solid phase extraction/ultra high performance liquid chromatography ( UHPLC)/tandem mass spectrometry( MS/MS)/amantadine/rimantadine/eggs/chickens分类
化学化工引用本文复制引用
林涛,樊建麟,刘兴勇,陈兴连,李彦刚,刘宏程..分散固相萃取净化-超高效液相色谱-串联质谱法测定鸡蛋和鸡肉中金刚烷胺和金刚乙胺残留[J].色谱,2015,(11):1169-1174,6.基金项目
云南省社会发展科技计划项目（2013RA012）；云南省应用基础研究计划青年项目（2013FD064）；云南省科技创新平台建设计划项目（2014DA001）． （）
