水生生物学报Issue(6)：1093-1099,7.DOI:10.7541/2015.144
CuSO4和ZnSO4对尼罗罗非鱼N-乙酰-β-D-氨基葡萄糖苷酶的影响
EFFECTS OF CuSO4 AND ZnSO4 ON N-ACETYL-β-D-GLUCOSAMINIDASE FROMOREOCHROMIS NILOTICUS
摘要
Abstract
N-Acetyl-β-D-glucosaminidase (EC 3.2.1.52, NAGase), hydrolyzing the oligomers of N-Acetyl-β-D-glucosamine (NAG) into monomer, is correlated with animal reproduction. In the current study, we in-vestigated the effect of two common drugs (CuSO4 and ZnSO4) on NAGase from spermary of Nile tilapia (Oreochromis niloticus). The results showed that theIC50 of CuSO4 and ZnSO4 were (1.23±0.05) mmol/L and (0.28±0.02) mmol/L, respectively. Both CuSO4 and ZnSO4 regulated the conformation of tilapia NAGase, which affected the fluorescence emission of the enzyme. The inhibitory activities of these two drugs were reversible, and the inhibition type of CuSO4 was noncompetitive but ZnSO4 was competitive. Both CuSO4 and ZnSO4 had significant influences on the thermal and pH stability of the enzyme. The results contribute to the use and control of CuSO4 and ZnSO4 in tilapia culture.关键词
尼罗罗非鱼/N-乙酰-β-D-氨基葡萄糖苷酶/CuSO4/ZnSO4Key words
Oreochromis niloticus/N-acetyl-β-D-glucosaminidase/CuSO4/ZnSO4分类
生物科学引用本文复制引用
张伟妮,谢璐娜,黄小红..CuSO4和ZnSO4对尼罗罗非鱼N-乙酰-β-D-氨基葡萄糖苷酶的影响[J].水生生物学报,2015,(6):1093-1099,7.基金项目
福建省科技重点项目(2012N0002)资助 （2012N0002）
