水生生物学报Issue(6)：1142-1149,8.DOI:10.7541/2015.150
鲁氏耶尔森氏菌口服微球疫苗特性分析及免疫效果研究
THE CHARACTERISTICS AND IMMUNE EFFICACY OF AN ORAL MICROSPHERES VACCINE OFYERSINIA RUCKERI
摘要
Abstract
Yersinia ruckeri is major pathogenic bacterium that is harmful to the aquaculture ofIctalurus punctatus. The conventional treatment such as antibiotics and chemicals often cause issues in, for example, food safety and drug resis-tance. The vaccine has been considered to be the most effective method of disease prevention and control. Currently, natural polymer materials have been used as the carrier for fish oral vaccine, and sodium alginate is regarded as an ideal material and has become a hotspot in research. In this study, we prepared the oral microspheres vaccine ofY. ruckeriby using formalin-inactivatedY. ruckeri as the antigen and sodium alginate as the carrier. We also studied the characteris-tics of this vaccine and evaluated its immune efficacy that was indicated by the serum non-specific immune parameters, the antibody titers and the relative survival rate. We found that the microspheres had a good form with a homogeneous distribution of the particle diameter; the average diameter was (8.76±1.73)μm and the span was 0.47; and the encapsu-lation efficiency of the vaccine was 94.51%. Moreover, this vaccine exhibited characteristics such as high acid resis-tance, good enteric solubility, and being safe. The activities of the serum lysozyme, the total superoxide disumutase (T-SOD) and the alternative pathway complement (ACH50) were significantly increased in the vaccinatedI. punctatus. The serum agglutination titers reached the peak value of 1: 8 in the 5th week, and the specific antibodies were detected in the 8th week after vaccination. The relative survival rate of theI. punctatus was 65.52%. In conclusion, the oral mi-crospheres vaccine ofY. ruckerieffectively protects the fish form theY. ruckeri disease.关键词
鲁氏耶尔森氏菌/口服疫苗/非特异性免疫功能/免疫保护Key words
Yersinia ruckeri/Oral vaccine/Non-specific immunity function/Relative percent survival分类
农业科技引用本文复制引用
阳磊,汪开毓,周燕,王二龙,王均,陈德芳,耿毅,赖为民..鲁氏耶尔森氏菌口服微球疫苗特性分析及免疫效果研究[J].水生生物学报,2015,(6):1142-1149,8.基金项目
教育部《长江学者和创新团队发展计划》创新团队专项(IRT0848) （IRT0848）
四川省科技厅产业链(2014NZ003) （2014NZ003）
四川省科技厅应用基础专项(2014JY0143)资助 （2014JY0143）
