水生态学杂志Issue(4)：86-91,6.DOI:10.15928/j.1674-3075.2015.04.012
泥鳅养殖水体中一株芽孢杆菌的筛选及其净水效果研究
Screening Bacillus subtilis to use for Improving Water Quality in Loach (Misgurnus anguillicaudatus)Culture Ponds
摘要
Abstract
The bacteria in probiotic preparations decompose organic matter to obtain energy for growth and repro-duction,thereby improving water quality at low cost and without producing harmful residuals.Thus,probiotics have been widely used in aquacultural waters to improve water quality.However,there are few reports on the screening, growth characterization and application of probiotics for culturing loach.To provide reliable probiotic strains for wa-ter quality improvement in loach (Misgurnus anguillicaudatus)culture ponds,we isolated and purified probiotic strains of native Bacillus from loach culture ponds.Four strains of Bacillus were obtained and a dominant strain,re-ferred to as Bacillus NQ1 ,was screened for removal of ammonia and nitrite nitrogen.Based on morphological fea-tures and physiological-biochemical characteristics,NQ1 was identified as Bacillus subtilis.Research on growth characteristics of Bacillus NQ1 indicates that the strain entered the logarithmic growth phase after 4 h and reached the stable growth stage after 16 h.A safety test of B.subtilis NQ1 was conducted in a loach pond with six groups designated:three NQ1 test groups with final pond concentrations of 105 ,106 and 107 cfu/mL;an Aeromonas hydro-philia group with a final concentration of 107 cfu/mL;an NQ1 plus A.hydrophilia group,both with final concen-trations of 107 cfu/mL;the control group that received sterile normal saline.Suspensions of the designated solutions were added to a loach culture pond and an equal volume of sterile normal saline solution was added to a pond as the control.Each group contained 20 loaches and tests were run in triplicate.During the test,aeration was continuous and fish were fed once daily.After 14 days,the survival rates for each group were calculated.Results show that loach feeding and activity in the three NQ1 test groups and the control group were normal and the loach survival rate in all four groups was 100%.The loach survival rate in the NQ1 plus A.hydrophilia group was only 65%,but still higher than the survival rate (40%)in the A.hydrophilia group.In the water purification test,ammonia and ni-trite nitrogen in the 1 ×107 cfu/mL NQ1 group decreased by 34.97% and 89.46% compared to the control group after 14 days.Therefore,B.subtilis NQ1 can improve water quality in aquaculture ponds and shows good potential as a probiotic for managing the microecology of loach culture ponds.关键词
芽孢杆菌/菌株筛选/水质净化/泥鳅养殖Key words
Bacillus/strain screening/water purification/loach (Misgurnus anguillicaudatus)breeding分类
农业科技引用本文复制引用
何沅滨,李义,陈亚军,凌去非,徐乐..泥鳅养殖水体中一株芽孢杆菌的筛选及其净水效果研究[J].水生态学杂志,2015,(4):86-91,6.基金项目
江苏省水产三新工程重大项目（DY2012-3-8）；江苏省水产三项工程项目（PJ2011-62）；江苏省科技厅项目（BE2012354）。 （）
